Teichoic acid content in different lineages of Staphylococcus aureus NCTC8325

A series of mec transformants of Staphylococcus aureus strain NCTC8325 were analysed for alterations in wall teichoic acid and lipoteichoic acid. Although the methicillin resistance determinant alters the autolytic behaviour of S. aureus, it had no effects on the cellular con tent, chain length, and alanine substitution of the lipoteichoic acid, or on the wall teichoic acid content and composition. However, independently of the presence or absence of the methicillin resistance determinant, level of methicillin resistance? or autolytic behaviour, a correlation was found between a 25% reduced cell wall phosphate content and either loss of prophages phi 11 and 13 or a 30-kb deletion in the chomosmal SmaI-F fragment adjacent to the prophage phi 11 attachment site.