Protection of the small intestinal clonogenic stem cells from radiation-induced damage by pretreatment with interleukin 11 also increases murine survival time

被引:74
作者
Potten, CS
机构
[1] CRC Department of Epithelial Biology, Paterson Inst. for Cancer Research, Christie Hospital NHS Trust, Manchester
[2] CRC Department of Epithelial Biology, Paterson Inst. for Cancer Research, Christie Hospital NHS Trust, Manchester, M20 9BX, Wilmslow Road
关键词
intestinal stem cells; clonogenic cells; radiation; 5-fluorouracil; survival time;
D O I
10.1002/stem.140452
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The effect of administering recombinant human interleukin 11 in conjunction with cytotoxic insults to the gastrointestinal tract has been studied using the crypt microcolony assay for stem cell function and whole-animal survival time studies. The cytotoxic regimens include single doses of gamma rays; single doses of 5-fluorouracil (5-FU) and multiple doses of 5-FU spared 6 h apart. Interleukin 11 (IL-11) (100 mu g/kg) delivered over a period of time prior to cytotoxic exposure afforded protection to the clonogenic cells in the crypts as seen with the microcolony assay and prolonged the animal survival time following radiation exposure. Continuing this dose of IL-11 after cytotoxic exposure afforded little additional protection. Three doses of 5-FU 6 h apart generated crypt survival curves similar to those obtained after a single dose of gamma rays. IL-ll given prior to two doses of 5-FU effectively abolished the cytotoxic effect of the second dose of 5-FU; i.e., 2.5-3.0 times more crypts survived if IL-II was administered when the higher 5-FU doses are considered. IL-11 given before a dose of 12 Gy of gamma rays prolonged the survival time of animals by three to four days. This confirms earlier studies demonstrating that protecting clonogenic cells in the crypt survival assay can result in beneficial effects on whole-animal survival times.
引用
收藏
页码:452 / 459
页数:8
相关论文
共 17 条
[1]   Effects of IL-11 on the growth of intestinal epithelial cells in vitro [J].
Booth, C ;
Potten, CS .
CELL PROLIFERATION, 1995, 28 (11) :581-594
[2]  
DU XX, 1994, BLOOD, V83, P33
[3]  
DU XX, 1994, BLOOD, V83, P2023
[4]   THE GASTROINTESTINAL SYNDROME AND MUCOSAL CLONOGENIC CELLS - RELATIONSHIPS BETWEEN TARGET-CELL SENSITIVITIES, LD50 AND CELL-SURVIVAL, AND THEIR MODIFICATION BY ANTIBIOTICS [J].
HENDRY, JH ;
POTTEN, CS ;
ROBERTS, NP .
RADIATION RESEARCH, 1983, 96 (01) :100-112
[5]  
KEITH JC, 1994, POLYFUNCTIONALITY HE, P79
[6]   GENOMIC SEQUENCE AND CHROMOSOMAL LOCATION OF HUMAN INTERLEUKIN-11 GENE (IL11) [J].
MCKINLEY, D ;
WU, QM ;
YANGFENG, T ;
YANG, YC .
GENOMICS, 1992, 13 (03) :814-819
[7]   MOLECULAR-CLONING OF A CDNA-ENCODING INTERLEUKIN-11, A STROMAL CELL-DERIVED LYMPHOPOIETIC AND HEMATOPOIETIC CYTOKINE [J].
PAUL, SR ;
BENNETT, F ;
CALVETTI, JA ;
KELLEHER, K ;
WOOD, CR ;
OHARA, RM ;
LEARY, AC ;
SIBLEY, B ;
CLARK, SC ;
WILLIAMS, DA ;
YANG, YC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (19) :7512-7516
[8]  
PETERSON RI, 1995, GASTROENTEROLOGY S1, V108, pA351
[9]  
Potten C, 1983, CYTOTOXIC INSULT TIS, V105-52
[10]  
Potten C. S., 1995, P45