The Mouse Blood-Brain Barrier Transcriptome: A New Resource for Understanding the Development and Function of Brain Endothelial Cells

被引：423

作者：

Daneman, Richard ^{[1
]}

Zhou, Lu ^{[2
]}

Agalliu, Dritan ^{[2
]}

Cahoy, John D. ^{[3
]}

Kaushal, Amit ^{[4
]}

Barres, Ben A. ^{[2
]}

机构：

[1] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA

[2] Stanford Univ, Dept Neurobiol, Sch Med, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Dev Biol, Sch Med, Stanford, CA 94305 USA

[4] Stanford Univ, Sch Med, Dept Biomed Informat, Stanford, CA 94305 USA

来源：

PLOS ONE | 2010年 / 5卷 / 10期

关键词：

TIGHT-JUNCTION PROTEIN; VASCULAR DEVELOPMENT; PHENOTYPIC HETEROGENEITY; PROGENITOR CELLS; GLIAL-CELLS; EXPRESSION; MICE; ANGIOGENESIS; PERMEABILITY; GENOMICS;

D O I：

10.1371/journal.pone.0013741

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The blood-brain barrier (BBB) maintains brain homeostasis and limits the entry of toxins and pathogens into the brain. Despite its importance, little is known about the molecular mechanisms regulating the development and function of this crucial barrier. In this study we have developed methods to highly purify and gene profile endothelial cells from different tissues, and by comparing the transcriptional profile of brain endothelial cells with those purified from the liver and lung, we have generated a comprehensive resource of transcripts that are enriched in the BBB forming endothelial cells of the brain. Through this comparison we have identified novel tight junction proteins, transporters, metabolic enzymes, signaling components, and unknown transcripts whose expression is enriched in central nervous system (CNS) endothelial cells. This analysis has identified that RXRalpha signaling cascade is specifically enriched at the BBB, implicating this pathway in regulating this vital barrier. This dataset provides a resource for understanding CNS endothelial cells and their interaction with neural and hematogenous cells.

引用

页数：16

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