Deactivation of gibberellin by 2-oxidation during germination of photoblastic lettuce seeds

被引:26
作者
Nakaminami, K
Sawada, Y
Suzuki, M
Kenmoku, H
Kawaide, H
Mitsuhashi, W
Sassa, T
Inoue, Y
Kamiya, Y
Toyomasu, T [1 ]
机构
[1] Iwate Univ, United Grad Sch Agr Sci, Course Sci Bioresources, Morioka, Iwate 0208550, Japan
[2] Yamagata Univ, Dept Bioresource Engn, Yamagata 9978555, Japan
[3] Tokyo Univ Agr & Technol, Dept Appl Biol Sci, Fuchu, Tokyo 1838509, Japan
[4] Univ Tokyo, Dept Appl Biol Sci, Noda, Chiba 2788510, Japan
[5] RIKEN, Plant Sci Ctr, Wako, Saitama 3510198, Japan
关键词
lettuce; gibberellin; deactivation; germination;
D O I
10.1271/bbb.67.1551
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gibberellin (GA) plays an important role in the induction of germination of photoblastic lettuce (Lactuca sativa L. cv. Grand Rapids) seeds. We have previously shown that gene expression of a GA 3-oxidase (Ls3h1) increased after a red light treatment, resulting in an increase in the endogenous content of GA(1), bioactive GA. Since the metabolism of GAs is also important for determining the endogenous levels of bioactive GAs, cDNAs encoding GA 2-oxidases (LsGA2ox1 and LsGA2ox2, for L. sativa GA 2-oxidase), which catalyze the deactivation of GAs, were isolated from lettuce seeds to investigate the regulation of these genes by light. An expression analysis shows that the mRNA levels of both enzymes was not markedly altered under different light conditions during germination. However, the amount of LsGA2ox2 transcripts was decreased to approximately half the level by red light. This reduction might play a role in the increase in GA(1) level by red light in the lettuce seeds.
引用
收藏
页码:1551 / 1558
页数:8
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