Autoradiographic analysis of dopamine receptor-stimulated [35S]GTPγS binding in rat striatum

被引：10

作者：

He, L ^{[1
]}

Di Monte, DA ^{[1
]}

Langston, JW ^{[1
]}

Quik, M ^{[1
]}

机构：

[1] Parkinsons Inst, Sunnyvale, CA 94089 USA

来源：

BRAIN RESEARCH | 2000年 / 885卷 / 01期

关键词：

autoradiography; dopamine receptor; rat; S-35]GTP gamma S binding; striatum;

D O I：

10.1016/S0006-8993(00)02981-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Autoradiographic analysis of [S-35]GTP gammaS binding was used to investigate functional activation of dopamine receptors in rat striatum. Dopamine-stimulated [S-35]GTP gammaS binding was observed with a maximal increase of 38% over basal activity. A similar stimulatory response was obtained with the D-2, agonist quinpirole, but not SKF-238393, a D-1, agonist. The effect of dopamine was blocked by the D-2 antagonist raclopride, but was unaffected by SCH-23990, a D-1, antagonist. There appeared to be a differential distribution of dopamine-stimulated [S-35]GTP gammaS binding, with the lowest activity obtained in the medial portion of the caudal striatum. These results demonstrate, using an autoradiographic approach, (i) that dopamine stimulated [S-35]GTP gammaS binding in the rat striatum occurs through activation of D-2, receptors, and (ii) that the effects of dopamine activation vary in different areas of the rat striatum. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：133 / 136

页数：4

共 22 条

[1] [S-35]GTP gamma S binding: A tool to evaluate functional activity of a cloned opioid receptor transiently expressed in COS cells [J].