Rapid Cellular Internalization of Multifunctional Star Polymers Prepared by Atom Transfer Radical Polymerization

被引：43

作者：

Cho, Hong Y. ^{[1
]}

Gao, Haifeng ^{[1
]}

Srinivasan, Abiraman ^{[2
]}

Hong, Joanna ^{[2
]}

Bencherif, Sidi A. ^{[1
]}

Siegwart, Daniel J. ^{[1
]}

Paik, Hyun-jong ^{[3
]}

Hollinger, Jeffrey O. ^{[2
]}

Matyjaszewski, Krzysztof ^{[1
]}

机构：

[1] Carnegie Mellon Univ, Dept Chem, Pittsburgh, PA 15213 USA

[2] Carnegie Mellon Univ, Dept Biomed Engn, Bone Tissue Engn Ctr, Pittsburgh, PA 15213 USA

[3] Pusan Natl Univ, Dept Polymer Sci & Engn, Pusan, South Korea

来源：

BIOMACROMOLECULES | 2010年 / 11卷 / 09期

基金：

美国国家科学基金会;

关键词：

CROSS-LINKING MACROMONOMERS; BLOCK-COPOLYMER; DELIVERY; CORE; ATRP; NANOPARTICLES; DENDRIMERS; VECTORS; BRUSHES; SYSTEMS;

D O I：

10.1021/bm1006272

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly(ethylene glycol) (PEG) star polymers containing GRGDS (Gly-Arg-Gly-Asp-Ser) peptide sequences on the star periphery were synthesized by atom transfer radical polymerization (ATRP) of poly(ethylene glycol) methyl ether methacrylate (PEGMA), GRGDS modified poly(ethylene glycol) acrylate (GRGDS-PEG-Acryl), fluorescein o-methacrylate (FMA), and ethylene glycol dimethacrylate (EGDMA) via an "arm-first" method. Star polymers were approximately 20 nm in diameter, as measured by dynamic light scattering and atomic force microscopy. Conjugation of FMA to the stars was confirmed by fluorescence microscopy, and successful attachment of GRGDS segments to the star periphery was confirmed by H-1 NMR spectroscopy. Both fluorescent PEG star polymers with and without peripheral GRGDS peptide segments were cultured with MC3T3-E1.4 cells. These star polymers were biocompatible with >= 90% cell viability after 24 h of incubation. Cellular uptake of PEG star polymers in MC3T3-E1.4 cells was observed by confocal microscopy. Rapid uptake of PEG star polymers with GRGDS peptides (similar to 100% of FITC-positive cells in 15 min measured by flow cytometry) was observed, suggesting enhanced delivery potential of these functional star polymers.

引用

页码：2199 / 2203

页数：5

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