Biochemical and genetic characterization of coagulin, a new antilisterial bacteriocin in the pediocin family of bacteriocins, produced by Bacillus coagulans I4

被引:150
作者
Le Marrec, C
Hyronimus, B
Bressollier, P
Verneuil, B
Urdaci, MC
机构
[1] Univ Bordeaux 1, Unite Secur Microbiol Aliments, ISTAB, F-33405 Talence, France
[2] ENITA Bordeaux, Lab Microbiol & Biochim Appl, F-33175 Gradignan, France
[3] IUT, Lab Genie Enzymat & Biovalorisat, Dept Genie Biol, F-87000 Limoges, France
关键词
D O I
10.1128/AEM.66.12.5213-5220.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A plasmid-linked antimicrobial peptide, named coagulin, produced by Bacillus coagulans I-4 has recently been reported (B. Hyronimus, C. Le Marrec and M. C. Urdaci, J. Appl. Microbiol. 85:42-50, 1998), In the present study, the complete, unambiguous primary amino acid sequence of the peptide was obtained by a combination of both N-terminal sequencing of purified peptide and the complete sequence deduced from the structural gene harbored by plasmid I-4. Data revealed that this peptide of 44 residues has an amino acid sequence similar to that described for pediocins AcH and PA-1, produced by different Pediococcus acidilactici strains and 100% identical. Coagulin and pediocin differed only by a single amino acid at their C terminus. Analysis of the genetic determinants revealed the presence, on the pI(4) DNA, of the entire 3.5-kb operon of four genes described for pediocin AcH and PA-1 production. No extended homology was observed between pSMB74 from P, acidilactici and pI(4) when analyzing the regions upstream and downstream of the operon, An oppositely oriented gene immediately dowstream of the bacteriocin operon specifies a 474-amino-acid protein which shows homology to Mob-Pre (plasmid recombination enzyme) proteins encoded by several small plasmids extracted from grampositive bacteria. This is the first report of a pediocin-like peptide appearing naturally in a non-lactic acid bacterium genus.
引用
收藏
页码:5213 / 5220
页数:8
相关论文
共 55 条
[1]   Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins [J].
Aymerich, T ;
Holo, H ;
Havarstein, LS ;
Hugas, M ;
Garriga, M ;
Nes, IF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (05) :1676-1682
[2]   Purification and amino acid sequences of piscicocins V1a and V1b, two class IIa bacteriocins secreted by Carnobacterium piscicola V1 that display significantly different levels of specific inhibitory activity [J].
BhugalooVial, P ;
Dousset, X ;
Metivier, A ;
Sorokine, O ;
Anglade, P ;
Boyaval, P ;
Marion, D .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (12) :4410-4416
[3]   PURIFICATION, CHARACTERIZATION AND ANTIMICROBIAL SPECTRUM OF A BACTERIOCIN PRODUCED BY PEDIOCOCCUS-ACIDILACTICI [J].
BHUNIA, AK ;
JOHNSON, MC ;
RAY, B .
JOURNAL OF APPLIED BACTERIOLOGY, 1988, 65 (04) :261-268
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   ULTRASTRUCTURE OF BACTERIOPHAGES AND BACTERIOCINS [J].
BRADLEY, DE .
BACTERIOLOGICAL REVIEWS, 1967, 31 (04) :230-+
[6]   PEDIOCIN PA-1, A BACTERIOCIN FROM PEDIOCOCCUS-ACIDILACTICI PAC1.0, FORMS HYDROPHILIC PORES IN THE CYTOPLASMIC MEMBRANE OF TARGET-CELLS [J].
CHIKINDAS, ML ;
GARCIAGARCERA, MJ ;
DRIESSEN, AJM ;
LEDEBOER, AM ;
NISSENMEYER, J ;
NES, IF ;
ABEE, T ;
KONINGS, WN ;
VENEMA, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (11) :3577-3584
[7]   ASSOCIATION OF A 13.6-MEGADALTON PLASMID IN PEDIOCOCCUS-PENTOSACEUS WITH BACTERIOCIN ACTIVITY [J].
DAESCHEL, MA ;
KLAENHAMMER, TR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1985, 50 (06) :1538-1541
[8]  
De Borjac H, 1974, ANN MICROBIOLOGIE B, V125, P529
[9]   STRUCTURAL ORGANIZATION OF PBC1, A CRYPTIC PLASMID FROM BACILLUS-COAGULANS [J].
DEROSSI, E ;
MILANO, A ;
BRIGIDI, P ;
BINI, F ;
RICCARDI, G .
JOURNAL OF BACTERIOLOGY, 1992, 174 (02) :638-642
[10]   MATURATION PATHWAY OF NISIN AND OTHER LANTIBIOTICS - POSTTRANSLATIONALLY MODIFIED ANTIMICROBIAL PEPTIDES EXPORTED BY GRAM-POSITIVE BACTERIA [J].
DEVOS, WM ;
KUIPERS, OP ;
VANDERMEER, JR ;
SIEZEN, RJ .
MOLECULAR MICROBIOLOGY, 1995, 17 (03) :427-437