Sequence analysis of a parvovirus B19 isolate and baculovirus expression of the non-structural protein

被引：28

作者：

Hicks, KE ^{[1
]}

Cubel, RCN ^{[1
]}

Cohen, BJ ^{[1
]}

Clewley, JP ^{[1
]}

机构：

[1] CENT PUBL HLTH LAB,DIV VIRUS REFERENCE,LONDON NW9 5HT,ENGLAND

来源：

ARCHIVES OF VIROLOGY | 1996年 / 141卷 / 07期

关键词：

D O I：

10.1007/BF01718833

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Serology for parvovirus B19 has been hampered by limited availability of antigen which has often had to be isolated from viraemic blood donations. We have determined the sequence of the genome of one such isolate (Stu). It is 99% similar to the sequences of two other isolates (Wi and Au) except at the far 5'-end, where it is more similar to the terminus of another isolate (Ala/Alb). Recombinant nonstructural protein, NS, was constructed. Antibodies to NS, as well as to the capsid proteins, VP1/2, were detected in patients with B19 infection.

引用

页码：1319 / 1327

页数：9

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