Specific immunohistochemical detection of cardiac/fetal alpha-actin in human cardiomyocytes and regenerating skeletal muscle cells

被引：27

作者：

Franke, WW ^{[1
]}

Stehr, S ^{[1
]}

Stumpp, S ^{[1
]}

Kuhn, C ^{[1
]}

Heid, H ^{[1
]}

Rackwitz, HR ^{[1
]}

Schnolzer, M ^{[1
]}

Baumann, R ^{[1
]}

Holzhausen, HJ ^{[1
]}

Moll, R ^{[1
]}

机构：

[1] UNIV HALLE WITTENBERG, INST PATHOL, D-06097 HALLE, GERMANY

来源：

DIFFERENTIATION | 1996年 / 60卷 / 04期

关键词：

D O I：

10.1046/j.1432-0436.1996.6040245.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We describe three murine monoclonal antibodies (mAbs) raised against a synthetic decapeptide representing the aminoterminal sequence of the cardiac/fetal isoform of sarcomeric alpha-actin. When used for immunoblotting or histological immunolocalization, these mAbs distinguish cardiac/fetal alpha-actin from skeletal muscle alpha-actin, and also from all other actin isoforms. We show, by immunofluorescence and immunoperoxidase microscopy of tissue sections, that cardiac/fetal alpha-actin can be localized not only in cardiomyocytes but also in skeletal muscles and their satellite cells during regeneration. These mAbs are potentially valuable in developmental biology, for the characterization of tissue and cultured myogenic cells, in patholoy, and for serodiagnosis.

引用

页码：245 / 250

页数：6

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