Direct measurement of the area expansion and shear moduli of the human red blood cell membrane skeleton

被引:137
作者
Lenormand, G
Hénon, S
Richert, A
Siméon, J
Gallet, F
机构
[1] CNRS, ESA 7057, Lab Biorheol & Hydrodynam Physicochim, F-75251 Paris 5, France
[2] Univ Paris 06, Paris, France
[3] Univ Paris 07, Paris, France
关键词
D O I
10.1016/S0006-3495(01)75678-0
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The area expansion and the shear moduli of the free spectrin skeleton, freshly extracted from the membrane of a human red blood cell (RBC), are measured by using optical tweezers micromanipulation. An RBC is trapped by three silica beads bound to its membrane. After extraction, the skeleton is deformed by applying calibrated forces to the beads. The area expansion modulus K, and shear modulus CL, of the two-dimensional spectrin network are inferred from the deformations measured as functions of the applied stress. In low hypotonic buffer (25 mOsm/kg), one finds K-C = 4.8 +/- 2.7 muN/m, mu (C) = 2.4 +/- 0.7 muN/m, and K-C/mu (C) = 1.9 +/- 1.0. In isotonic buffer, one measures higher values for K-C, mu (C), and K-C/mu (C), partly because the skeleton collapses in a high-ionic-strength environment. Some data concerning the time evolution of the mechanical properties of the skeleton after extraction and the influence of ATP are also reported. In the Discussion, it is shown that the measured values are consistent with estimates deduced from experiments carried out on the intact membrane and agree with theoretical and numerical predictions concerning two-dimensional networks of entropic springs.
引用
收藏
页码:43 / 56
页数:14
相关论文
共 34 条
[1]   FLUCTUATIONS OF SOLID MEMBRANES [J].
ARONOVITZ, JA ;
LUBENSKY, TC .
PHYSICAL REVIEW LETTERS, 1988, 60 (25) :2634-2637
[2]  
BENNETT V, 1993, ANNU REV CELL BIOL, V9, P27, DOI 10.1146/annurev.cb.09.110193.000331
[3]   COMPUTER-SIMULATION OF A MODEL NETWORK FOR THE ERYTHROCYTE CYTOSKELETON [J].
BOAL, DH .
BIOPHYSICAL JOURNAL, 1994, 67 (02) :521-529
[4]   NEGATIVE POISSON RATIO IN 2-DIMENSIONAL NETWORKS UNDER TENSION [J].
BOAL, DH ;
SEIFERT, U ;
SHILLCOCK, JC .
PHYSICAL REVIEW E, 1993, 48 (06) :4274-4283
[5]   Alteration of α-spectrin ubiquitination due to age-dependent changes in the erythrocyte membrane [J].
Corsi, D ;
Paiardini, M ;
Crinelli, R ;
Bucchini, A ;
Magnani, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 261 (03) :775-783
[6]   MOLECULAR MAPS OF RED-CELL DEFORMATION - HIDDEN ELASTICITY AND IN-SITU CONNECTIVITY [J].
DISCHER, DE ;
MOHANDAS, N ;
EVANS, EA .
SCIENCE, 1994, 266 (5187) :1032-1035
[7]   Simulations of the erythrocyte cytoskeleton at large deformation. II. Micropipette aspiration [J].
Discher, DE ;
Boal, DH ;
Boey, SK .
BIOPHYSICAL JOURNAL, 1998, 75 (03) :1584-1597
[8]   ON THE MEASUREMENT OF SHEAR ELASTIC-MODULI AND VISCOSITIES OF ERYTHROCYTE PLASMA-MEMBRANES BY TRANSIENT DEFORMATION IN HIGH-FREQUENCY ELECTRIC-FIELDS [J].
ENGELHARDT, H ;
SACKMANN, E .
BIOPHYSICAL JOURNAL, 1988, 54 (03) :495-508
[10]   NEW MEMBRANE CONCEPT APPLIED TO ANALYSIS OF FLUID SHEAR-DEFORMED AND MICROPIPET-DEFORMED RED BLOOD-CELLS [J].
EVANS, EA .
BIOPHYSICAL JOURNAL, 1973, 13 (09) :941-954