Purification and characterization of an esterase from Micrococcus sp YGJ1 hydrolyzing phthalate esters

被引：52

作者：

Akita, K ^{[1
]}

Naitou, C ^{[1
]}

Maruyama, K ^{[1
]}

机构：

[1] Gifu Univ, Fac Engn, Dept Biomol Sci, Gifu 5011193, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2001年 / 65卷 / 07期

关键词：

esterase; phthalate esters; Micrococcus;

D O I：

10.1271/bbb.65.1680

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An esterase hydrolyzing phthalate esters has been purified from Micrococcus sp. YGJ1. The enzyme, a monomeric protein (M-r=56 kDa) with a pl of 4.0, hydrolyzes various aliphatic and aromatic carboxylesters. The medium chain (C-3-C-4) esters are the most preferred substrates. The enzyme is inhibited by HgCl2 and p-chloromercuribenzoate but not by phenylmethylsulfonyl fluoride.

引用

页码：1680 / 1683

页数：4

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