PPARγ ligands inhibit TNF-α-induced LOX-1 expression in cultured endothelial cells

被引：48

作者：

Chiba, Y ^{[1
]}

Ogita, T ^{[1
]}

Ando, K ^{[1
]}

Fujita, T ^{[1
]}

机构：

[1] Univ Tokyo, Sch Med, Dept Internal Med, Bunkyo Ku, Tokyo 1138655, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 286卷 / 03期

关键词：

lectin-like oxidized low-density lipoprotein; receptor-1; peroxisome proliferator-activated receptors; tumor necrosis factor-alpha; phorbol; 12-myristrate; 13-acetate; atherosclerosis; inflammation;

D O I：

10.1006/bbrc.2001.5361

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Endothelial dysfunction or activation, elicited by oxidized low-density lipoprotein (OxLDL), has been implicated in the initiation and progression of atherosclerosis. We elucidated whether tumor necrosis factor-alpha (TNF-alpha)-induced endothelial OxLDL receptor, lectinlike OxLDL receptor-1 (LOX-1), mRNA expression is modified by peroxisome proliferator-activated receptor (PPAR) activators in cultured bovine aortic endothelial cells (BAEC). We confirmed that both PPAR alpha and PPAR-gamma were expressed in BAEC by reverse transcription-polymerase chain reaction analysis. Natural PPAR gamma ligand 15-deoxy-Delta (12,14)-prostaglandin J(2) (15d-PGJ(2)) and the thiazolidinediones, pioglitazone and troglitazone, decreased TNF-alpha -induced LOX-1 mRNA expression in BAEC. LOX-1 expression induced by phorbol 12-myristrate 13-acetate was also inhibited by 15d-PGJ2. In contrast, PPAR alpha ligands, Wy14643 and fenofibric acid, did not alter TNF-alpha -induced LOX-1 expression. TNF-a-induced immunohistochemical staining of LOX-1 was suppressed by 15d-PGJ2 but not Wy14643. Taken together, PPAR gamma activators inhibit TNF-a-induced LOX-1 expression in cultured BAEC, which may beneficially influence inflammatory responses in atherosclerosis. (C) 2001 Academic Press.

引用

页码：541 / 546

页数：6

共 30 条

[1] Structure and chromosomal assignment of the human lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1) gene [J].

Aoyama, T ;

Sawamura, T ;

Furutani, Y ;

Matsuoka, R ;

Yoshida, MC ;

Fujiwara, H ;

Masaki, T .

BIOCHEMICAL JOURNAL, 1999, 339 :177-184

[2] Activation of proliferator-activated receptors α and γ induces apoptosis of human monocyte-derived macrophages [J].

Chinetti, G ;

Griglio, S ;

Antonucci, M ;

Torra, IP ;

Delerive, P ;

Majd, Z ;

Fruchart, JC ;

Chapman, J ;

Najib, J ;

Staels, B .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (40) :25573-25580

[3] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[4] Oxidized low density lipoprotein (ox-LDL) binding to ox-LDL receptor-1 in endothelial cells induces the activation of NF-κB through an increased production of intracellular reactive oxygen species [J].

Cominacini, L ;

Fratta Pasini, A ;

Garbin, U ;

Davoli, A ;

Tosetti, ML ;

Campagnola, M ;

Rigoni, A ;

Pastorino, AM ;

Lo Cascio, V ;

Sawamura, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (17) :12633-12638

[5] Peroxisome proliferator-activated receptor activators inhibit thrombin-induced endothelin-1 production in human vascular endothelial cells by inhibiting the activator protein-1 signaling pathway [J].

Delerive, P ;

Martin-Nizard, F ;

Chinetti, G ;

Trottein, F ;

Fruchart, JC ;

Najib, J ;

Duriez, P ;

Staels, B .

CIRCULATION RESEARCH, 1999, 85 (05) :394-402

[6] The PPAR alpha-leukotriene B-4 pathway to inflammation control [J].

Devchand, PR ;

Keller, H ;

Peters, JM ;

Vazquez, M ;

Gonzalez, FJ ;

Wahli, W .

NATURE, 1996, 384 (6604) :39-43

[7] Hypolipidemic drugs, polyunsaturated fatty acids, and eicosanoids are ligands for peroxisome proliferator-activated receptors alpha and delta [J].

Forman, BM ;

Chen, J ;

Evans, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (09) :4312-4317

[8]

GIMBRONE MA, 1995, ANN NY ACAD SCI, V748, P122

[9] Peroxisome proliferator-activated receptor-γ ligands inhibit nitric oxide synthesis in vascular smooth muscle cells [J].

Ikeda, U ;

Shimpo, M ;

Murakami, Y ;

Shimada, K .

HYPERTENSION, 2000, 35 (06) :1232-1236

[10] Thiazolidinediones down-regulate plasminogen activator inhibitor type 1 expression in human vascular endothelial cells:: A possible role for PPARγ in endothelial function [J].

Kato, K ;

Satoh, H ;

Endo, Y ;

Yamada, D ;

Midorikawa, S ;

Sato, W ;

Mizuno, K ;

Fujita, T ;

Tsukamoto, K ;

Watanabe, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 258 (02) :431-435

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