Genotoxicity study on nicotine and nicotine-derived nitrosamine by accelerator mass spectrometry

被引：34

作者：

Li, XS ^{[1
]}

Wang, HF ^{[1
]}

Shi, JY ^{[1
]}

Wang, XY ^{[1
]}

Liu, YF ^{[1
]}

Li, K ^{[1
]}

Lu, XY ^{[1
]}

Wang, JJ ^{[1
]}

Liu, KX ^{[1
]}

Guo, ZY ^{[1
]}

机构：

[1] BEIJING UNIV,INST HEAVY ION PHYS,BEIJING 100871,PEOPLES R CHINA

来源：

RADIOCARBON | 1996年 / 38卷 / 02期

关键词：

D O I：

10.1017/S0033822200017677

中图分类号：

P3 [地球物理学]; P59 [地球化学];

学科分类号：

0708 ; 070902 ;

摘要：

We have studied DNA adduction with C-14-labeled nicotine and nicotine-derived nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), by accelerator mass spectrometry (AMS) in mouse liver at doses equivalent to low-level exposure of humans. The dose ranges of nicotine and NNK administered were from 0.4 mu g to 4.0 x 10(2) mu g kg b.w.(-1), and from 0.1 mu g to 2.0 x 10(4) mu g kg b.w.(-1), respectively. In the exposure of mice to either nicotine or NNK, the number of DNA adducts increased linearly with increasing dose. The detection limit of DNA adducts was 1 adduct per 10(11) nucleotide molecules. This limit is 1-4 orders of magnitude lower than that of other techniques used for quantification of DNA adducts. The results of our animal experiments enabled us to speculate that nicotine is a potential carcinogen. According to the procedure for C-14-labeled-NNK synthesis, we discuss the ultimate chemical speciation of NNK bound to DNA. From the animal tests we derived a directly perceivable relation between tobacco consumption and DNA adduction as the carcinogenic risk assessment.

引用

页码：347 / 353

页数：7

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