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Cryptococcus neoformans senses CO2 through the carbonic anhydrase Can2 and the adenylyl cyclase Cac1
被引:123
作者:

Mogensen, EG
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机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England

Janbon, G
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机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England

Chaloupka, J
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机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England

Steegborn, C
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机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England

Fu, MS
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机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England

Moyrand, F
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机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England

Klengel, T
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机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England

Pearson, DS
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机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England

Geeves, MA
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机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England

Buck, J
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机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England

Levin, LR
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h-index: 0
机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England

Mühlschlegel, FA
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机构: Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England
机构:
[1] Univ Kent, Dept Biosci, Canterbury CT2 7NJ, Kent, England
[2] Inst Pasteur, CNRS, FRE2849, Unite Mycol Mol, F-75015 Paris, France
[3] Cornell Univ, Joan & Sanford Weill Med Coll 1, Dept Pharmacol, New York, NY 10021 USA
[4] Ruhr Univ Bochum, Dept Physiol Chem, D-44801 Bochum, Germany
基金:
英国惠康基金;
关键词:
D O I:
10.1128/EC.5.1.103-111.2006
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Cryptococcus neoformans, a fungal pathogen of humans, causes fatal meningitis in immunocompromised patients. Its virulence is mainly determined by the elaboration of a polysaccharide capsule surrounding its cell wall. During its life, C. neoformans is confronted with and responds to dramatic variations in CO2 concentrations; one important morphological change triggered by the shift from its natural habitat (0.033% CO2) to infected hosts (5% CO2) is the induction of capsule biosynthesis. In cells, CO2 is hydrated to bicarbonate in a spontaneous reaction that is accelerated by carbonic anhydrases. Here we show that C. neoformans contains two beta-class carbonic anhydrases, Can1 and Can2. We further demonstrate that CAN2, but not CAN], is abundantly expressed and essential for the growth of C. neoformans in its natural environment, where CO2 concentrations are limiting. Structural studies reveal that Can2 forms a homodimer in solution. Our data reveal Can2 to be the main carbonic anhydrase and suggest a physiological role for bicarbonate during C. neoformans growth. Bicarbonate directly activates the C. neoformans Cac1 adenylyl cyclase required for capsule synthesis. We show that this specific activation is optimal at physiological pH.
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页码:103 / 111
页数:9
相关论文
共 37 条
[1]
Cryptococcosis in children with AIDS
[J].
Abadi, J
;
Nachman, S
;
Kressel, AB
;
Pirofski, L
.
CLINICAL INFECTIOUS DISEASES,
1999, 28 (02)
:309-313

Abadi, J
论文数: 0 引用数: 0
h-index: 0
机构: Albert Einstein Coll Med, Dept Microbiol & Immunol, Div Infect Dis, Bronx, NY 10461 USA

Nachman, S
论文数: 0 引用数: 0
h-index: 0
机构: Albert Einstein Coll Med, Dept Microbiol & Immunol, Div Infect Dis, Bronx, NY 10461 USA

Kressel, AB
论文数: 0 引用数: 0
h-index: 0
机构: Albert Einstein Coll Med, Dept Microbiol & Immunol, Div Infect Dis, Bronx, NY 10461 USA

Pirofski, L
论文数: 0 引用数: 0
h-index: 0
机构: Albert Einstein Coll Med, Dept Microbiol & Immunol, Div Infect Dis, Bronx, NY 10461 USA
[2]
Adenylyl cyclase functions downstream of the Gα protein Gpa1 and controls mating and pathogenicity of Cryptococcus neoformans
[J].
Alspaugh, JA
;
Pukkila-Worley, R
;
Harashima, T
;
Cavallo, LM
;
Funnell, D
;
Cox, GM
;
Perfect, JR
;
Kronstad, JW
;
Heitman, J
.
EUKARYOTIC CELL,
2002, 1 (01)
:75-84

Alspaugh, JA
论文数: 0 引用数: 0
h-index: 0
机构:
Duke Univ, Med Ctr 3555, Dept Genet, Durham, NC 27710 USA Duke Univ, Med Ctr 3555, Dept Genet, Durham, NC 27710 USA

Pukkila-Worley, R
论文数: 0 引用数: 0
h-index: 0
机构: Duke Univ, Med Ctr 3555, Dept Genet, Durham, NC 27710 USA

Harashima, T
论文数: 0 引用数: 0
h-index: 0
机构: Duke Univ, Med Ctr 3555, Dept Genet, Durham, NC 27710 USA

Cavallo, LM
论文数: 0 引用数: 0
h-index: 0
机构: Duke Univ, Med Ctr 3555, Dept Genet, Durham, NC 27710 USA

Funnell, D
论文数: 0 引用数: 0
h-index: 0
机构: Duke Univ, Med Ctr 3555, Dept Genet, Durham, NC 27710 USA

Cox, GM
论文数: 0 引用数: 0
h-index: 0
机构: Duke Univ, Med Ctr 3555, Dept Genet, Durham, NC 27710 USA

Perfect, JR
论文数: 0 引用数: 0
h-index: 0
机构: Duke Univ, Med Ctr 3555, Dept Genet, Durham, NC 27710 USA

Kronstad, JW
论文数: 0 引用数: 0
h-index: 0
机构: Duke Univ, Med Ctr 3555, Dept Genet, Durham, NC 27710 USA

Heitman, J
论文数: 0 引用数: 0
h-index: 0
机构: Duke Univ, Med Ctr 3555, Dept Genet, Durham, NC 27710 USA
[3]
The gene NCE103 (YNL036w) from Saccharomyces cerevisiae encodes a functional carbonic anhydrase and its transcription is regulated by the concentration of inorganic carbon in the medium
[J].
Amoroso, G
;
Morell-Avrahov, L
;
Müller, D
;
Klug, K
;
Sültemeyer, D
.
MOLECULAR MICROBIOLOGY,
2005, 56 (02)
:549-558

Amoroso, G
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Kaiserslautern, Dept Biol, D-67653 Kaiserslautern, Germany Univ Kaiserslautern, Dept Biol, D-67653 Kaiserslautern, Germany

Morell-Avrahov, L
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Kaiserslautern, Dept Biol, D-67653 Kaiserslautern, Germany Univ Kaiserslautern, Dept Biol, D-67653 Kaiserslautern, Germany

Müller, D
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Kaiserslautern, Dept Biol, D-67653 Kaiserslautern, Germany Univ Kaiserslautern, Dept Biol, D-67653 Kaiserslautern, Germany

Klug, K
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Kaiserslautern, Dept Biol, D-67653 Kaiserslautern, Germany Univ Kaiserslautern, Dept Biol, D-67653 Kaiserslautern, Germany

Sültemeyer, D
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Kaiserslautern, Dept Biol, D-67653 Kaiserslautern, Germany Univ Kaiserslautern, Dept Biol, D-67653 Kaiserslautern, Germany
[4]
Carbonic anhydrase and CO2 sensing during Cryptocloccus neoformans growth, differentiation, and virulence
[J].
Bahn, YS
;
Cox, GM
;
Perfect, JR
;
Heitman, J
.
CURRENT BIOLOGY,
2005, 15 (22)
:2013-2020

Bahn, YS
论文数: 0 引用数: 0
h-index: 0
机构: Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA

Cox, GM
论文数: 0 引用数: 0
h-index: 0
机构: Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA

Perfect, JR
论文数: 0 引用数: 0
h-index: 0
机构: Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA

Heitman, J
论文数: 0 引用数: 0
h-index: 0
机构:
Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA
[5]
A yeast under cover:: the capsule of Cryptococcus neoformans
[J].
Bose, I
;
Reese, AJ
;
Ory, JJ
;
Janbon, G
;
Doering, TL
.
EUKARYOTIC CELL,
2003, 2 (04)
:655-663

Bose, I
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Untie Mycol Mol, F-75724 Paris, France

Reese, AJ
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Untie Mycol Mol, F-75724 Paris, France

Ory, JJ
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Untie Mycol Mol, F-75724 Paris, France

Janbon, G
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Untie Mycol Mol, F-75724 Paris, France

Doering, TL
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Untie Mycol Mol, F-75724 Paris, France
[6]
Multiple sequence alignment with the Clustal series of programs
[J].
Chenna, R
;
Sugawara, H
;
Koike, T
;
Lopez, R
;
Gibson, TJ
;
Higgins, DG
;
Thompson, JD
.
NUCLEIC ACIDS RESEARCH,
2003, 31 (13)
:3497-3500

Chenna, R
论文数: 0 引用数: 0
h-index: 0
机构: ULP, CNRS, INSERM,Inst Genet & Biol Mol & Cellulaire, Lab Biol & Genom Struct, F-67404 Illkirch Graffenstaden, France

Sugawara, H
论文数: 0 引用数: 0
h-index: 0
机构: ULP, CNRS, INSERM,Inst Genet & Biol Mol & Cellulaire, Lab Biol & Genom Struct, F-67404 Illkirch Graffenstaden, France

Koike, T
论文数: 0 引用数: 0
h-index: 0
机构: ULP, CNRS, INSERM,Inst Genet & Biol Mol & Cellulaire, Lab Biol & Genom Struct, F-67404 Illkirch Graffenstaden, France

Lopez, R
论文数: 0 引用数: 0
h-index: 0
机构: ULP, CNRS, INSERM,Inst Genet & Biol Mol & Cellulaire, Lab Biol & Genom Struct, F-67404 Illkirch Graffenstaden, France

Gibson, TJ
论文数: 0 引用数: 0
h-index: 0
机构: ULP, CNRS, INSERM,Inst Genet & Biol Mol & Cellulaire, Lab Biol & Genom Struct, F-67404 Illkirch Graffenstaden, France

Higgins, DG
论文数: 0 引用数: 0
h-index: 0
机构: ULP, CNRS, INSERM,Inst Genet & Biol Mol & Cellulaire, Lab Biol & Genom Struct, F-67404 Illkirch Graffenstaden, France

Thompson, JD
论文数: 0 引用数: 0
h-index: 0
机构: ULP, CNRS, INSERM,Inst Genet & Biol Mol & Cellulaire, Lab Biol & Genom Struct, F-67404 Illkirch Graffenstaden, France
[7]
Crystal structure of E-coli β-carbonic anhydrase, an enzyme with an unusual pH-dependent activity
[J].
Cronk, JD
;
Endrizzi, JA
;
Cronk, MR
;
O'Neill, JW
;
Zhang, KYJ
.
PROTEIN SCIENCE,
2001, 10 (05)
:911-922

Cronk, JD
论文数: 0 引用数: 0
h-index: 0
机构: Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA

Endrizzi, JA
论文数: 0 引用数: 0
h-index: 0
机构: Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA

Cronk, MR
论文数: 0 引用数: 0
h-index: 0
机构: Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA

O'Neill, JW
论文数: 0 引用数: 0
h-index: 0
机构: Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA

Zhang, KYJ
论文数: 0 引用数: 0
h-index: 0
机构: Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA
[8]
ConSurf: Identification of Functional Regions in Proteins by Surface-Mapping of Phylogenetic Information
[J].
Glaser, F
;
Pupko, T
;
Paz, I
;
Bell, RE
;
Bechor-Shental, D
;
Martz, E
;
Ben-Tal, N
.
BIOINFORMATICS,
2003, 19 (01)
:163-164

Glaser, F
论文数: 0 引用数: 0
h-index: 0
机构: Tel Aviv Univ, George S Wise Fac Life Sci, IL-69978 Tel Aviv, Israel

Pupko, T
论文数: 0 引用数: 0
h-index: 0
机构: Tel Aviv Univ, George S Wise Fac Life Sci, IL-69978 Tel Aviv, Israel

Paz, I
论文数: 0 引用数: 0
h-index: 0
机构: Tel Aviv Univ, George S Wise Fac Life Sci, IL-69978 Tel Aviv, Israel

Bell, RE
论文数: 0 引用数: 0
h-index: 0
机构: Tel Aviv Univ, George S Wise Fac Life Sci, IL-69978 Tel Aviv, Israel

Bechor-Shental, D
论文数: 0 引用数: 0
h-index: 0
机构: Tel Aviv Univ, George S Wise Fac Life Sci, IL-69978 Tel Aviv, Israel

Martz, E
论文数: 0 引用数: 0
h-index: 0
机构: Tel Aviv Univ, George S Wise Fac Life Sci, IL-69978 Tel Aviv, Israel

Ben-Tal, N
论文数: 0 引用数: 0
h-index: 0
机构:
Tel Aviv Univ, George S Wise Fac Life Sci, IL-69978 Tel Aviv, Israel Tel Aviv Univ, George S Wise Fac Life Sci, IL-69978 Tel Aviv, Israel
[9]
VIRULENCE OF CRYPTOCOCCUS-NEOFORMANS - REGULATION OF CAPSULE SYNTHESIS BY CARBON-DIOXIDE
[J].
GRANGER, DL
;
PERFECT, JR
;
DURACK, DT
.
JOURNAL OF CLINICAL INVESTIGATION,
1985, 76 (02)
:508-516

GRANGER, DL
论文数: 0 引用数: 0
h-index: 0

PERFECT, JR
论文数: 0 引用数: 0
h-index: 0

DURACK, DT
论文数: 0 引用数: 0
h-index: 0
[10]
The "Soluble" adenylyl cyclase in sperm mediates multiple signaling events required for fertilization
[J].
Hess, KC
;
Jones, BH
;
Marquez, B
;
Chen, YQ
;
Ord, TS
;
Kamenetsky, M
;
Miyamoto, C
;
Zippin, JH
;
Kopf, GS
;
Suarez, SS
;
Levin, LR
;
Williams, CJ
;
Buck, J
;
Moss, SB
.
DEVELOPMENTAL CELL,
2005, 9 (02)
:249-259

Hess, KC
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Jones, BH
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Marquez, B
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Chen, YQ
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Ord, TS
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Kamenetsky, M
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Miyamoto, C
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Zippin, JH
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Kopf, GS
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Suarez, SS
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Levin, LR
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Williams, CJ
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Buck, J
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA

Moss, SB
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Joan & Sanford Weill Med Coll, Grad Sch Med Sci, Dept Pharmacol, New York, NY 10021 USA