Fluorescent amplifying recognition for DNA G-quadruplex folding with a cationic conjugated polymer: A platform for homogeneous potassium detection

被引：406

作者：

He, F ^{[1
]}

Tang, YL ^{[1
]}

Wang, S ^{[1
]}

Li, YL ^{[1
]}

Zhu, DB ^{[1
]}

机构：

[1] Chinese Acad Sci, Key Lab Organ Solids, Inst Chem, Beijing 100080, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 35期

关键词：

D O I：

10.1021/ja051507i

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Single-stranded DNA with G-rich sequences can fold into secondary structures, G-quadruplexes, via intramolecular hydrogen-bonding interactions. This conformational change can be detected by a homogeneous assay method based on fluorescence resonance energy transfer (FRET) from a water-soluble cationic conjugated polymer (CCP) to a fluorescein chromophore labeled at the terminus of the G-quadruplex DNA. The space charge density around the DNA controls the efficiency of FRET from the CCP to the fluorescein. The higher FRET efficiency for the CCP/G-quadruplex pair is correlated to the stronger electrostatic interactions between the more condensed G-quadruplex and the CCP in comparison to the CCP/ssDNA pair. Since the potassium ion can specifically bind to the G-quadruplex DNA, the G-quartet-DNA/CCPs assembly can also be used as a platform to sense the potassium ion in water with high selectivity and sensitivity.

引用

页码：12343 / 12346

页数：4

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