HVJ-based non-viral gene transfer method: successful gene therapy using HGF and VEGF genes in experimental ischemia

被引:10
作者
Shimamura, M
Sato, N
Yoshimura, S
Kaneda, Y
Morishita, R
机构
[1] Osaka Univ, Grad Sch Med, Div Clin Gene Therapy, Suita, Osaka 5650871, Japan
[2] Osaka Univ, Grad Sch Med, Div Gene Therapy Sci, Suita, Osaka, Japan
[3] Univ Tokyo, Grad Sch Med, Dept Adv Clin Sci & Therapeut, Tokyo, Japan
[4] Gifu Univ, Sch Med, Dept Neurosurg, Gifu 500, Japan
来源
FRONTIERS IN BIOSCIENCE-LANDMARK | 2006年 / 11卷
关键词
brain; nervous system; treatment; therapeutics; gene therapy; HVJ-liposome; HVJ-envelope; cerebral ischemia; HGF; VEGF; review;
D O I
10.2741/1833
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
VEGF and HGF are pleiotropic factors that regulate cell growth, cell motility, and morphogenesis of various types of cells. The receptors of these growth factors are expressed in neurons and endothelial cells, and are identified as neurotrophic, neuroprotective, and angiogenic factors. Indeed, gene therapy using viral vectors encoding the VEGF or HGF gene has been reported to be effective for preventing the expansion of ischemic injury. However, the safety issue of viral vectors is a major problem in clinical application. To overcome this problem, we have developed an HVJ-based non-viral vector, which achieves high-efficiency transfection rates of viral vectors with the safety of liposomes. This review discusses the feasibility of gene therapy using an HVJ-based non-viral vector containing the VEGF or HGF gene for cerebral ischemia.
引用
收藏
页码:753 / 759
页数:7
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