Clinical significance of CMV-specific T helper responses in lung transplant recipients

被引:17
作者
Zeevi, A
Morel, P
Spichty, K
Dauber, J
Yousem, S
Williams, P
Grgurich, W
Pham, S
Iacono, A
Keenan, R
Duquesnoy, R
Griffith, B
机构
[1] Univ Pittsburgh, Med Ctr, Thomas E Starzl Transplantat Inst, Div Transplantat Pathol, Pittsburgh, PA 15261 USA
[2] Univ Pittsburgh, Med Ctr, Thomas E Starzl Transplantat Inst, Div Transplant Surg, Pittsburgh, PA 15261 USA
[3] Univ Pittsburgh, Med Ctr, Thomas E Starzl Transplantat Inst, Div Med, Pittsburgh, PA 15261 USA
关键词
D O I
10.1016/S0198-8859(98)00088-3
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. Cytomegalovirus (CMV) disease continues to be a major problem for lung transplant patients who generate an inefficient immune response to control this viral infection. Both T helper and cytotoxic T cells are thought to play an important role in prevention and control of CMV disease. We investigated the clinical significance of CMV-specific memory responses in lung transplant recipients. Method. Peripheral blood samples (140) were collected from 99 lung transplant recipients. Patients were grouped according to their pre-transplant CMV serological status as recipient/donor (R-/D+, 25 patients), 28 R+/D+ patients, 35 R+/D- patients and 11 R-/D- patients. Memory responses to CMV whole antigen, 5 CMV proteins, and tetanus toxoid (TT) were measured in a 6-day proliferative assay. Results were expressed as the stimulation index (SI = experimental cpm/background cpm), and were considered positive ii the SI was >3 and the cpm values were over 1,000. Results. The frequency of positive CMV memory responses was similar in three groups: 64% for R-/D+, 63% for R+/D+ and 56% for R+/D- except for R-/D- (21%). The memory response to TT was similar for all four groups (70% of samples were positive). The level of responsiveness to individual CMV proteins was much higher in R+/D+ group (65%) than the other two groups (35% for R+/D-, and 31% for R-/D+). We determined the temporal relationship between the presence of CMV-specific memory responses and the diagnosis of CMV disease. In the R-/D+ group, 16 of 17 patients who had CMV disease eventually developed CMV-specific memory. In those patients (n = 3) who failed to develop CMV-specific T helper response for a prolonged time, all had recurrent CMV disease. In the R+/D+ group, 4 of 8 patients with CMV disease exhibited CMV-specific memory responses. Three of 4 patients in whom we observed a persistent absence of CMV-specific memory had multiple episodes of CMV pneumonitis. In the R+/D- group, only one of 4 patients with CMV disease had suppressed CMV-specific memory response after first episode of CMV pneumonitis and had recurrent disease. Conclusion. In lung transplant recipients, the loss or persistent lack of CMV-specific memory following infection was associated with chronic CMV disease. These data suggest that monitoring T helper memory responses following primary CMV infection or after augmented immunosuppression for treatment of rejection may identify those patients at risk for morbidity associated with recurrent CMV disease. Human Immunology 59, 768-775 (1998). (C) American Society for Histocompatibility and Immunogenetics, 1998. Published by Elsevier Science Inc.
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页码:768 / 775
页数:8
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