Asymmetric tethering of flat and curved lipid membranes by a golgin

被引：146

作者：

Drin, Guillaume ^{[1
,2
]}

Morello, Vincent ^{[1
,2
]}

Casella, Jean-Francois ^{[1
,2
]}

Gounon, Pierre ^{[3
]}

Antonny, Bruno ^{[1
,2
]}

机构：

[1] Univ Nice, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France

[2] CNRS, F-06560 Valbonne, France

[3] Ctr Commun Microscopie Appl, F-06108 Nice, France

来源：

SCIENCE | 2008年 / 320卷 / 5876期

关键词：

D O I：

10.1126/science.1155821

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Golgins, long stringlike proteins, tether cisternae and transport vesicles at the Golgi apparatus. We examined the attachment of golgin GMAP-210 to lipid membranes. GMAP- 210 connected highly curved liposomes to flatter ones. This asymmetric tethering relied on motifs that sensed membrane curvature both in the N terminus of GMAP- 210 and in ArfGAP1, which controlled the interaction of the C terminus of GMAP- 210 with the small guanine nucleotide-binding protein Arf1. Because membrane curvature constantly changes during vesicular trafficking, this mode of tethering suggests a way to maintain the Golgi architecture without compromising membrane flow.

引用

页码：670 / 673

页数：4

共 22 条

[1] Molecular basis for Golgi maintenance and biogenesis [J].

Altan-Bonnet, N ;

Sougrat, R ;

Lippincott-Schwartz, J .

CURRENT OPINION IN CELL BIOLOGY, 2004, 16 (04) :364-372

[2] Close membrane-membrane proximity induced by Ca2+-dependent multivalent binding of synaptotagmin-1 to phospholipids [J].

Araç, D ;

Chen, XC ;

Khant, HA ;

Ubach, J ;

Ludtke, SJ ;

Kikkawa, M ;

Johnson, AE ;

Chiu, W ;

Südhof, TC ;

Rizo, J .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2006, 13 (03) :209-217

[3] ArfGAP1 responds to membrane curvature through the folding of a lipid packing sensor motif [J].

Bigay, J ;

Casella, JF ;

Drin, G ;

Mesmin, B ;

Antonny, B .

EMBO JOURNAL, 2005, 24 (13) :2244-2253

[4] Lipid packing sensed by ArfGAP1 couples COPI coat disassembly to membrane bilayer curvature [J].

Bigay, J ;

Gounon, P ;

Robineau, S ;

Antonny, B .

NATURE, 2003, 426 (6966) :563-566

[5] Kinetics of DNA-mediated docking reactions between vesicles tethered to supported lipid bilayers [J].

Chan, Yee-Hung M. ;

Lenz, Peter ;

Boxer, Steven G. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (48) :18913-18918

[6] A human exchange factor for ARF contains Sec7- and pleckstrin-homology domains [J].

Chardin, P ;

Paris, S ;

Antonny, B ;

Robineau, S ;

BeraudDufour, S ;

Jackson, CL ;

Chabre, M .

NATURE, 1996, 384 (6608) :481-484

[7] A general amphipathic α-helical motif for sensing membrane curvature [J].

Drin, Guillaume ;

Casella, Jean-Francois ;

Gautier, Romain ;

Boehmer, Thomas ;

Schwartz, Thomas U. ;

Antonny, Bruno .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2007, 14 (02) :138-146

[8] COPI domains required for coatomer integrity, and novel interactions with ARF and ARF-GAP [J].

Eugster, A ;

Frigerio, G ;

Dale, M ;

Duden, R .

EMBO JOURNAL, 2000, 19 (15) :3905-3917

[9] The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi [J].

Gillingham, AK ;

Tong, AHY ;

Boone, C ;

Munro, S .

JOURNAL OF CELL BIOLOGY, 2004, 167 (02) :281-292

[10] Arf GAPs and their interacting proteins [J].

Inoue, Hiroki ;

Randazzo, Paul A. .

TRAFFIC, 2007, 8 (11) :1465-1475

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