Use of genome selected repeated sequences increases the sensitivity of PCR detection of Tropheryma whipplei

被引：72

作者：

Fenollar, F ^{[1
]}

Fournier, PE ^{[1
]}

Robert, C ^{[1
]}

Raoult, D ^{[1
]}

机构：

[1] Univ Mediterranee, Fac Med, Unite Rickettsies, CNRS,UMR 6020, F-13385 Marseille 05, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 01期

关键词：

D O I：

10.1128/JCM.42.1.401-403.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The availability of the Tropheryma whipplei genome offers the putative possibility of choosing logical DNA targets. We applied a PCR assay (targeting repeated sequences of T. whipplei) to samples from patients with Whipple's disease and to those from members of a control group. When compared to the results seen with regular PCR, the sensitivity of repeat PCR was significantly enhanced (P = 0.02) without alteration of its specificity.

引用

页码：401 / 403

页数：3

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