Determination of bioactive diterpenoids from Andrographics paniculata by micellar electrokinetic chromatography

被引：70

作者：

Cheung, HY ^{[1
]}

Cheung, CS ^{[1
]}

Kong, CK ^{[1
]}

机构：

[1] City Univ Hong Kong, Res Grp Bioact Prod, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 930卷 / 1-2期

关键词：

Andrographics paniculata; andrographolide; deoxyandrographolide; neoandrographolide;

D O I：

10.1016/S0021-9673(01)01160-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The present paper describes the development of a micellar electrokinetic chromatographic (MEKC) method for simultaneous determination of andrographolide, deoxyandrographolide and neoandrographolide in ethanol extracts of Andrographis paniculata. Separations were carried out in a fused-silica capillary tube with UV detection at 214 nm. Good separation was achieved using a 20 mM borate buffer, containing 20 mM sodium dodecyl sulphate and 10 mM sodium cholate, adjusted to pH 8.3 at an operating voltage of 25 kV, temperature of 35 degreesC and a hydrodynamic injection of 5 s. The method was validated with good correlation coefficients obtained (0.9986-0.9989) while relative standard deviation (RSD) of migration time was between 1.14 and 2.42. It is concluded that this method could be used for speedy and accurate qualitative and quantitative analysis of bioactive diterpenoids in andrographis herb and its derived products. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：171 / 176

页数：6

共 19 条

[1]

ALTRIA KD, 1996, CAPILLARY ELECTROPHO, P29

[2]

[Anonymous], HUAZUE TONGBO

[3]

Borhanuddin M, 1994, Bangladesh Med Res Counc Bull, V20, P24

[4]

CHANG RS, 1991, P SOC EXP BIOL MED, V197, P59

[5]

CHEUNG HY, 2001, PHARM J, V10, P23

[6]

CHEUNG N, 1995, CHUNG TSAO YAO, V26, P191

[7]

How to achieve higher repeatability and reproducibility in capillary electrophoresis [J].

Faller, T ;

Engelhardt, H .

JOURNAL OF CHROMATOGRAPHY A, 1999, 853 (1-2) :83-94

[8]

Fu Xintong, 1995, Zhongguo Zhongyao Zazhi, V20, P351

[9]

HANDA SS, 1990, INDIAN J MED RES-B, V92, P284

[10]

HANDA SS, 1990, INDIAN J MED RES-B, V92, P276

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