Recurrence of autoreactive antigen-specific CD4+T cells in autoimmune diabetes after pancreas transplantation

被引：53

作者：

Laughlin, Elsa ^{[1
]}

Burke, George ^{[2
,5
]}

Pugliese, Alberto ^{[2
,3
,4
]}

Falk, Ben ^{[1
]}

Nepom, Gerald ^{[1
,6
]}

机构：

[1] Benaroya Res Inst Virginia Mason, Seattle, WA 98101 USA

[2] Univ Miami, Miller Sch Med, Diabet Res Inst, Miami, FL 33136 USA

[3] Univ Miami, Miller Sch Med, Dept Med, Miami, FL 33136 USA

[4] Univ Miami, Miller Sch Med, Dept Microbiol Immunol, Miami, FL 33136 USA

[5] Univ Miami, Miller Sch Med, Dept Surg, Div Transplantat, Miami, FL 33136 USA

[6] Univ Washington, Sch Med, Seattle, WA USA

来源：

CLINICAL IMMUNOLOGY | 2008年 / 128卷 / 01期

关键词：

autoimmune disease; pancreas transplantation; type; 1; diabetes; GAD65; MHC class II tetramer; CD4 T cells; islet replacement therapy;

D O I：

10.1016/j.clim.2008.03.459

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

CD4+ T cells specific for the diabetes-associated autoantigen GAD65 were analyzed using peripheral blood samples after pancreas transplantation in subjects with T1D with clinical evidence of recurrent autoimmune diabetes. MHC class II tetramers facilitated the identification and cloning of antigen-specific autoreactive cells, which were found at several time points over a multiyear span, in spite of chronic immunosuppression of the subjects. Comparisons of TCR clonotypes by cDNA sequencing revealed that identical T cells were present in the circulation, separated by tong time intervals, consistent with a persistent memory response associated with recurrent autoimmunity. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：23 / 30

页数：8

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