Determination of the rheological properties of Matrigel for optimum seeding conditions in microfluidic cell cultures

被引：32

作者：

Kane, K. I. W. ^{[1
]}

Moreno, E. Lucumi ^{[1
]}

Lehr, C. M. ^{[2
]}

Hachi, S. ^{[1
]}

Dannert, R. ^{[2
]}

Sanctuary, R. ^{[2
]}

Wagner, C. ^{[2
,3
]}

Fleming, R. M. T. ^{[1
]}

Baller, J. ^{[2
]}

机构：

[1] Univ Luxembourg, Luxembourg Ctr Syst Biomed, 7 Ave Hauts Fourneaux, L-4362 Esch Sur Alzette, Luxembourg

[2] Univ Luxembourg, Lab Phys Adv Mat, Phys & Mat Sci Res Unit, 162A Ave Faiencerie, L-1511 Luxembourg, Luxembourg

[3] Saarland Univ, Dept Expt Phys, Campus E2-6, D-66123 Saarbrucken, Germany

来源：

AIP ADVANCES | 2018年 / 8卷 / 12期

基金：

欧盟地平线“2020”;

关键词：

CROSS-LINKING POLYMER; HYDROGELS;

D O I：

10.1063/1.5067382

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

Hydrogels are increasingly used as a surrogate extracellular matrix in three-dimensional cell culture systems, including microfluidic cell culture. Matrigel is a hydrogel of natural origin widely used in cell culture, particularly in the culture of stem cell-derived cell lines. The use of Matrigel as a surrogate extracellular matrix in microfluidic systems is challenging due to its biochemical, biophysical, and biomechanical properties. Therefore, understanding and characterising these properties is a prerequisite for optimal use of Matrigel in microfluidic systems. We used rheological measurements and particle image velocimetry to characterise the fluid flow dynamics of liquefied Matrigel during loading into a three-dimensional microfluidic cell culture device. Using fluorescence microscopy and fluorescent beads for particle image velocimetry measurements (velocity profiles) in combination with classical rheological measurements of Matrigel (viscosity versus shear rate), we characterised the shear rates experienced by cells in a microfluidic device for three-dimensional cell culture. This study provides a better understanding of the mechanical stress experienced by cells, during seeding of a mixture of hydrogel and cells, into three-dimensional microfluidic cell culture devices. (C) 2018 Author(s).

引用

页数：11

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