Biotin-specific synthetic receptors prepared using molecular imprinting

被引：54

作者：

Piletska, E ^{[1
]}

Piletsky, S ^{[1
]}

Karim, K ^{[1
]}

Terpetschnig, E ^{[1
]}

Turner, A ^{[1
]}

机构：

[1] Cranfield Univ, Cranfield Ctr Analyt Sci, Inst Biosci & Technol, Cranfield MK45 4DT, Beds, England

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 504卷 / 01期

关键词：

biotin; computational design; molecularly imprinted polymer; photografting polymerisation;

D O I：

10.1016/S0003-2670(03)00813-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The composition of new molecularly imprinted polymers (MIPs) specific for biotin was optimised using molecular modelling software. Three functional monomers: methacrylic acid (MAA), 2-(trifluoromethyl)acrylic acid (TFAA) and 2-acrylamido-2-methylpropanesulfonic acid (AMPSA), which demonstrated the highest binding scores with biotin, were tested on their ability to generate specific binding sites. The imprinted polymers were photografted to the surface of polystyrene microspheres in water. The affinity of the synthetic "receptor" sites was evaluated in binding experiments using horseradish peroxidase-labelled biotin. Good correlation was found between the modelling results and the performance of the materials in the template re-binding study. The dissociation constants for all MIPs were 1.4-16.8 nM, which is sufficient for most analytical applications where biotin is used as a label. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：179 / 183

页数：5

共 8 条

[1] Rational design of a polymer specific for microcystin-LR using a computational approach [J].