Non-flammable preparative reversed-phase liquid chromatography of recombinant human insulin-like growth factor-I

被引：8

作者：

Fahrner, RL ^{[1
]}

Lester, PM ^{[1
]}

Blank, GS ^{[1
]}

Reifsnyder, DH ^{[1
]}

机构：

[1] Genentech Inc, Dept Recovery Sci, S San Francisco, CA 94080 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 830卷 / 01期

关键词：

growth factors; mobile phase composition; hexylene glycol;

D O I：

10.1016/S0021-9673(98)00866-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Acetonitrile is used as an eluent for reversed-phase chromatography. However, because it is a flammable solvent, using acetonitrile on a large scale requires expensive equipment and facilities specially designed for flammable solvents. Using a non-flammable solvent as an eluent eliminates this expense. A method was developed to purify recombinant human insulin-like growth factor I by reversed-phase high-performance liquid chromatography using gradient elution with hexylene glycol, a non-flammable replacement for acetonitrile. The separation produced equivalent yield, purity and throughput as reversed-phase chromatography using elution with acetonitrile. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：127 / 134

页数：8

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