The 3' end of yeast 5.8S rRNA is generated by an exonuclease processing mechanism

被引:165
作者
Mitchell, P
Petfalski, E
Tollervey, D
机构
[1] Europ. Molecular Biology Laboratory
关键词
rRNA; RNA processing; 3'->5' exonuclease; RRP4; gene; S-cerevisiae;
D O I
10.1101/gad.10.4.502
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Eukaryotic rRNAs (with the exception of 5S rRNA) are synthesized from a contiguous pre-rRNA precursor by a complex series of processing reactions. Final maturation of yeast 5.8S rRNA involves processing of a 3'-extended, 7S precursor that contains similar to 140 nucleotides of the internal transcribed spacer 2 (ITS2) region. In yeast strains carrying the temperature-sensitive (ts) rrp4-1 mutation, 5.8S rRNA species were observed with 3' extensions of variable length extending up to the 3' end of the 7S pre-rRNA. These 3'-extended 5.8S rRNA species were observed at low levels in rrp4-1 strains under conditions permissive for growth and increased in abundance upon transfer to the nonpermissive temperature. The RRP4 gene was cloned by complementation of the ts growth phenotype of rrp4-1 strains. RRP4 encodes an essential protein of 39-kD predicted molecular mass. Immunoprecipitated Rrp4p exhibited a 3' --> 5' exoribonuclease activity in vitro that required RNA with a 3'-terminal hydroxyl group and released nucleoside 5' monophosphates. We conclude that the 7S pre-rRNA is processed to 5.8S rRNA by a 3' --> 5' exonuclease activity involving Rrp4p. Homologs of Rrp4p are found in both humans and the fission yeast Schizosaccaromyces pombe (43% and 52% identity, respectively), suggesting that the mechanism of 5.8S rRNA 3' end formation has been conserved throughout eukaryotes.
引用
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页码:502 / 513
页数:12
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