E2Fs regulate the expression of genes involved in differentiation, development, proliferation, and apoptosis

被引:560
作者
Müller, H
Bracken, AP
Vernell, R
Moroni, MC
Christians, F
Grassilli, E
Prosperini, E
Vigo, E
Oliner, JD
Helin, K [1 ]
机构
[1] European Inst Oncol, Dept Expt Oncol, I-20141 Milan, Italy
[2] Affymetrix Inc, Santa Clara, CA 95051 USA
关键词
E2F; retinoblastoma; microarray; target gene bias;
D O I
10.1101/gad.864201
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The retinoblastoma protein (pRB) and its two relatives, p107 and p130, regulate development and cell proliferation in part by inhibiting the activity of E2F-regulated promoters. We have used high-density oligonucleotide arrays to identify genes in which expression changed in response to activation of E2F1, E2F2, and E2F3. We show that the E2Fs control the expression of several genes that are involved in cell proliferation. We also show that the E2Fs regulate a number of genes involved in apoptosis, differentiation, and development. These results provide possible genetic explanations to the variety of phenotypes observed as a consequence of a deregulated pRB/E2F pathway.
引用
收藏
页码:267 / 285
页数:19
相关论文
共 61 条
[1]  
Abell M.L., 1999, Statistics with Mathematica
[2]  
Ausubel F.M., 1988, CURRENT PROTOCOLS MO
[3]   Sox9 is required for cartilage formation [J].
Bi, WM ;
Deng, JM ;
Zhang, ZP ;
Behringer, RR ;
de Crombrugghe, B .
NATURE GENETICS, 1999, 22 (01) :85-89
[4]   The Maf transcription factors: regulators of differentiation [J].
Blank, V ;
Andrews, NC .
TRENDS IN BIOCHEMICAL SCIENCES, 1997, 22 (11) :437-441
[5]   Fibroblast growth factor receptors: lessons from the genes [J].
Burke, D ;
Wilkes, D ;
Blundell, TL ;
Malcolm, S .
TRENDS IN BIOCHEMICAL SCIENCES, 1998, 23 (02) :59-62
[6]   Retinoblastoma protein positively regulates terminal adipocyte differentiation through direct interaction with C/EBPs [J].
Chen, PL ;
Riley, DJ ;
Chen, YM ;
Lee, WH .
GENES & DEVELOPMENT, 1996, 10 (21) :2794-2804
[7]   Shared role of the pRB-related p130 and p107 proteins in limb development [J].
Cobrinik, D ;
Lee, MH ;
Hannon, G ;
Mulligan, G ;
Bronson, RT ;
Dyson, N ;
Harlow, E ;
Beach, D ;
Weinberg, RA ;
Jacks, T .
GENES & DEVELOPMENT, 1996, 10 (13) :1633-1644
[8]   Expression analysis with oligonucleotide microarrays reveals that MYC regulates genes involved in growth, cell cycle, signaling, and adhesion [J].
Coller, HA ;
Grandori, C ;
Tamayo, P ;
Colbert, T ;
Lander, ES ;
Eisenman, RN ;
Golub, TR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (07) :3260-3265
[9]   Human and murine PTX1/Ptx1 gene maps to the region for Treacher Collins Syndrome [J].
Crawford, MJ ;
Lanctot, C ;
Tremblay, JJ ;
Jenkins, N ;
Gilbert, D ;
Copeland, N ;
Beatty, B ;
Drouin, J .
MAMMALIAN GENOME, 1997, 8 (11) :841-845
[10]   Activin A promotes progenitor differentiation into photoreceptors in rodent retina [J].
Davis, AA ;
Matzuk, MM ;
Reh, TA .
MOLECULAR AND CELLULAR NEUROSCIENCE, 2000, 15 (01) :11-21