Cleavage of human immunoglobulin G by Treponema denticola

被引:6
作者
Grenier, D
Mayrand, D
机构
[1] Univ Laval, Fac Med Dent, Grp Rech Ecol Buccale, Quebec City, PQ G1K 7P4, Canada
[2] Univ Laval, Fac Sci & Genie, Quebec City, PQ G1K 7P4, Canada
基金
英国医学研究理事会;
关键词
Treponema denticola; periodontitis; virulence; immunoglobulin; protease; glycosidase;
D O I
10.1006/anae.2000.0361
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mechanisms by which microbial proteases may counteract the local host immune system include the degradation of immunoglobulins. In this study, we report the capacity of the periodontopathogen Treponema denticola to degrade immunoglobulin G (IgG). Intact IgG was not hydrolysed by whole cells, as revealed by SDS-PAGE analysis. When IgG molecules were treated with endoglycosidase F to remove the carbohydrate moiety, significant degradation was observed. However, pre-treatment with glycosidases possessing specificities different from endoglycosidase F (lysozyme or neuraminidase) did not render the molecule susceptible to cleavage by T. denticola. SDS-PAGE analysis of the IgG degradation products suggests that T. denticola cleaves inside the heavy chain polypeptide. Serine-specific protease inhibitors were highly effective in inhibiting the degradation of glycosidase-treated IgG molecules by T. denticola. The synergistic effect of glycolytic enzymes and T. denticola proteases on IgG may occur during periodontitis since both glycolytic activities and spirochete numbers significantly increase in diseased periodontal sites. (C) 2001 Academic Press.
引用
收藏
页码:1 / 4
页数:4
相关论文
共 18 条
[1]   GLYCOSIDASE ACTIVITIES IN GINGIVAL CREVICULAR FLUID IN SUBJECTS WITH ADULT PERIODONTITIS OR GINGIVITIS [J].
BEIGHTON, D ;
RADFORD, JR ;
NAYLOR, MN .
ARCHIVES OF ORAL BIOLOGY, 1992, 37 (05) :343-348
[2]   METABOLIC COOPERATION IN ORAL MICROBIAL COMMUNITIES DURING GROWTH ON MUCIN [J].
BRADSHAW, DJ ;
HOMER, KA ;
MARSH, PD ;
BEIGHTON, D .
MICROBIOLOGY-UK, 1994, 140 :3407-3412
[3]   THE PROTEIN-COMPOSITION OF GINGIVAL CREVICULAR FLUID SAMPLED FROM MALE-ADOLESCENTS WITH NO DESTRUCTIVE PERIODONTITIS - BASE-LINE DATA OF A LONGITUDINAL-STUDY [J].
CURTIS, MA ;
STERNE, JAC ;
PRICE, SJ ;
GRIFFITHS, GS ;
COULTHURST, SK ;
WILTON, JMA ;
JOHNSON, NW .
JOURNAL OF PERIODONTAL RESEARCH, 1990, 25 (01) :6-16
[4]   IMMUNOGLOBULIN-DEGRADING ENZYMES IN LOCALIZED JUVENILE PERIODONTITIS [J].
GREGORY, RL ;
KIM, DE ;
KINDLE, JC ;
HOBBS, LC ;
LLOYD, DR .
JOURNAL OF PERIODONTAL RESEARCH, 1992, 27 (03) :176-183
[5]  
Grenier Daniel, 2000, P275
[6]   Inability of intact cells of Treponema denticola to degrade human serum proteins IgA, IgG and albumin [J].
Hollmann, R ;
Van der Hoeven, HJS .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1999, 26 (07) :477-479
[7]  
HOLT S C, 1991, Critical Reviews in Oral Biology and Medicine, V2, P177
[8]   Characterization of the Treponema denticola prtP gene encoding a prolyl-phenylalanine-specific protease (Dentilisin) [J].
Ishihara, K ;
Miura, T ;
Kuramitsu, HK ;
Okuda, K .
INFECTION AND IMMUNITY, 1996, 64 (12) :5178-5186
[9]   DEGRADATION OF IMMUNOGLOBULIN-G BY PERIODONTAL BACTERIA [J].
JANSEN, HJ ;
VANDERHOEVEN, JS ;
KIEBOOM, CWA ;
GOERTZ, JHC ;
CAMP, PJM .
ORAL MICROBIOLOGY AND IMMUNOLOGY, 1994, 9 (06) :345-351