Relocating expression of vegetative insecticidal protein into mother cell of Bacillus thuringiensis

被引：12

作者：

Arora, N ^{[1
]}

Selvapandiyan, A ^{[1
]}

Agrawal, N ^{[1
]}

Bhatnagar, RK ^{[1
]}

机构：

[1] Int Ctr Genet Engn & Biotechnol, New Delhi 110067, India

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 310卷 / 01期

关键词：

Bt-toxin relocation; vegetative insecticidal protein; sporulation-linked expression;

D O I：

10.1016/j.bbrc.2003.08.137

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vegetative insecticidal protein (VIP) is a class of insecticidal proteins produced by some strains of Bacillus thuringiensis during the vegetative stage of their growth. Unlike delta-endotoxins which are produced as parasporal inclusion bodies within the cell during sporulation, VIP is secreted into the culture medium. Here we report the relocation of the expression of VIP into the mother cell compartment in a manner similar to well-characterized Cry proteins. Relocation of VIP is directed to mother cell by placing its synthesis under sporulation-dependent promoters, BtI and BtII. The insertion of cry preferred transcription termination sequence at the 3' region and a STAB-SD sequence at the 5' region of the gene provided stability to the vip transcript and enhanced its yield. The demonstrated expression of VIP within the cells in the form of inclusion bodies would facilitate development of a suitable formulation for the application of this class of insecticidal proteins in the field. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：158 / 162

页数：5

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