Evaluation of pan-dermatophyte nested PCR in diagnosis of onychomycosis

被引:46
作者
Garg, Jaya
Tilak, Ragini
Singh, Sanjay
Gulati, Anil Kumar
Garg, Atul
Prakash, Pradyot
Nath, Gopal
机构
[1] Banaras Hindu Univ, Inst Med Sci, Dept Microbiol, Varanasi 221005, Uttar Pradesh, India
[2] Banaras Hindu Univ, Inst Med Sci, Dept Dermatol, Varanasi 221005, Uttar Pradesh, India
关键词
D O I
10.1128/JCM.02367-06
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase I gene (CHS1) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis. Results indicate that nested PCR may be considered the gold standard for the diagnosis of cases of onychomycosis for which the etiological agents are dermatophytes.
引用
收藏
页码:3443 / 3445
页数:3
相关论文
共 10 条
[1]  
Arca E, 2004, EUR J DERMATOL, V14, P52
[2]   Facing up to the diagnostic uncertainty and management of onychomycoses [J].
Arrese, JE ;
Piérard-Franchimont, C ;
Piérard, GE .
INTERNATIONAL JOURNAL OF DERMATOLOGY, 1999, 38 :1-6
[3]   Detection and differentiation of causative fungi of onychomycosis using PCR amplification and restriction enzyme analysis [J].
Baek, SC ;
Chae, HJ ;
Houh, D ;
Byun, DG ;
Cho, BK .
INTERNATIONAL JOURNAL OF DERMATOLOGY, 1998, 37 (09) :682-686
[4]  
DAWSON B, 2001, BASIC CLIN BIOSTATIS, P147
[5]   FORTNIGHTLY REVIEW - FUNGAL NAIL DISEASE - A GUIDE TO GOOD PRACTICE (REPORT OF A WORKING GROUP OF THE BRITISH-SOCIETY-FOR-MEDICAL-MYCOLOGY) [J].
DENNING, DW ;
EVANS, EGV ;
KIBBLER, CC ;
RICHARDSON, MD ;
ROBERTS, MM ;
ROGERS, TR ;
WARNOCK, DW ;
WARREN, RE .
BMJ-BRITISH MEDICAL JOURNAL, 1995, 311 (7015) :1277-1281
[6]   Onychomycosis: Pathogenesis, diagnosis, and management [J].
Elewski, BE .
CLINICAL MICROBIOLOGY REVIEWS, 1998, 11 (03) :415-+
[7]  
Greenberg RS, 1996, MED EPIDEMIOLOGY, V3rd, P77
[8]  
Kano R, 2003, J VET MED SCI, V65, P267, DOI 10.1292/jvms.65.267
[9]   Initial symptoms of Parkinson's disease with elderly onset [J].
Nagayama, H ;
Hamamoto, M ;
Nito, C ;
Takagi, S ;
Miyazaki, T ;
Katayama, Y .
GERONTOLOGY, 2000, 46 (03) :129-132
[10]   Onychomycosis: therapeutic update [J].
Scher, RK .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1999, 40 (06) :S21-S26