Expression of a chitinase transgene in rose (Rosa hybrida L) reduces development of blackspot disease (Diplocarpon rosae Wolf)

被引:94
作者
Marchant, R [1 ]
Davey, MR
Lucas, JA
Lamb, CJ
Dixon, RA
Power, JB
机构
[1] Univ Nottingham, Dept Life Sci, Plant Genet Manipulat Grp, Nottingham NG7 2RD, England
[2] Univ Bristol, Long Ashton Res Stn, IACR, Dept Agr Sci, Bristol BS18 9AF, Avon, England
[3] Salk Inst Biol Studies, Div Plant Biol, La Jolla, CA 92037 USA
[4] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73402 USA
基金
英国生物技术与生命科学研究理事会;
关键词
chitinase; Diplocarpon rosae; disease resistance; genetic engineering; Rosa hybrida L;
D O I
10.1023/A:1009642707505
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Blackspot, caused by the Ascomycete fungus Diplocarpon rosae, is the most widespread and pernicious disease of cultivated roses. While some species of rose possess resistance to D. rosae, none of the modern-day rose cultivars are fully resistant to the pathogen. In the current study, Biolistic gene delivery was used to introduce a rice gene, encoding a basic (Class I), chitinase into embryogenic callus of the blackspot-susceptible rose (Rosa hybrida L.) cv. Glad Tidings. The plasmid used for transformation carried the neomycin phosphotransferase (nptII) gene facilitating the selection and regeneration of transgenic plants on medium containing 250 mg/l kanamycin. Southern analysis confirmed integration of 2-6 copies of the chitinase gene into the rose genome; gene expression was confirmed by enzyme assay. Bioassays demonstrated that expression of the chitinase transgene reduced the severity of blackspot development by 13-43%. This degree of resistance to the pathogen correlated with the level of chitinase expression in the transgenic rose plants. The introduction of disease defence genes into rose provides a method of producing blackspot-resistant rose cultivars sought by breeders and growers.
引用
收藏
页码:187 / 194
页数:8
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