Immobilization of glucose oxidase within calcium alginate gel capsules

被引：159

作者：

Blandino, A ^{[1
]}

Macías, M ^{[1
]}

Cantero, D ^{[1
]}

机构：

[1] Univ Cadiz, Fac Sci, Dept Chem Engn Food Technol & Environm Technol, Biol & Enzymat Reactors Res Grp, Puerto Real, Cadiz, Spain

来源：

PROCESS BIOCHEMISTRY | 2001年 / 36卷 / 07期

关键词：

glucose; glucose oxidase; calcium alginate; capsules; gelation conditions; kinetics;

D O I：

10.1016/S0032-9592(00)00240-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucose oxidase (GOD) was encapsulated within calcium alginate gel capsules. The effects of gelation conditions on capsule characteristics such as thickness. percentage of enzyme leakage and encapsulation efficiency were studied and the optimal conditions for GOD encapsulation obtained. Oxidation of glucose to gluconic acid followed Michaelis-Menten kinetics. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：601 / 606

页数：6

共 23 条

[1] Glucose oxidase release from calcium alginate gel capsules
Blandino, A
Macías, M
Cantero, D
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 2000, 27 (3-5) : 319 - 324
[2] Formation of calcium alginate gel capsules:: Influence of sodium alginate and CaCl2 concentration on gelation kinetics
Blandino, A
Macías, M
Cantero, D
[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 88 (06) : 686 - 689
[3] Bulmus V, 1997, CHEM ENG J, V65, P71, DOI 10.1016/S0923-0467(96)03156-9
[4] Chang HN, 1996, BIOTECHNOL BIOENG, V51, P157, DOI 10.1002/(SICI)1097-0290(19960720)51:2<157::AID-BIT4>3.0.CO
[5] 2-I
[6] Chang TMS, 1996, CAN J PHYSL PHARM, V44, P115
[7] GACESA P, 1987, ENZYME TECHNOLOGY, P65
[8] Gerhartz W, 1990, ENZYMES IND PRODUCTI, P77
[9] BIOLOGICAL INTERACTIONS BETWEEN POLYSACCHARIDES AND DIVALENT CATIONS - EGG-BOX MODEL
GRANT, GT
MORRIS, ER
REES, DA
SMITH, PJC
THOM, D
[J]. FEBS LETTERS, 1973, 32 (01) : 195 - 198
[10] C-13-NMR STUDIES OF MONOMERIC COMPOSITION AND SEQUENCE IN ALGINATE
GRASDALEN, H
LARSEN, B
SMIDSROD, O
[J]. CARBOHYDRATE RESEARCH, 1981, 89 (02) : 179 - 191

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