Highly sensitive northern hybridization using a rapid protocol for downward alkaline blotting of RNA

被引:35
作者
Ingelbrecht, IL [1 ]
Mandelbaum, CI [1 ]
Mirkov, TE [1 ]
机构
[1] Texas A&M Univ, Texas Agr Expt Stn, Weslaco, TX 78596 USA
关键词
D O I
10.2144/98253st03
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple and fast RNA gel blot procedure is described that uses 50 mM NaOH to simultaneously transfer and fix RNA to a positively charged nylon membrane. The RNA is transferred in a downward direction, and transfer is routinely completed within 2.5 h. The resulting blots give increased sensitivity over existing methods without affecting RNA integrity and can be used in both radioactive and nonradioactive detection procedures.
引用
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页码:420 / +
页数:4
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