Epitope mapping and specificity of the anti-α-synuclein monoclonal antibody Syn-1 in mouse brain and cultured cell lines

被引:61
作者
Perrin, RJ
Payton, JE
Barnett, DH
Wraight, CL
Woods, WS
Ye, L
George, JM
机构
[1] Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA
[2] Univ Illinois, Med Scholars Program, Urbana, IL 61801 USA
[3] Univ Illinois, Dept Cell & Struct Biol, Urbana, IL 61801 USA
关键词
synuclein; immunoreactivity; Parkinson's disease; dopaminergic; breast cancer; knockout;
D O I
10.1016/S0304-3940(03)00781-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
While alpha- and beta-synuclein largely overlap in their expression in the vertebrate brain, only alpha-synuclein accumulates in the fibrillar aggregates typical of Parkinson's disease. It is thus critical to have immunological reagents that distinguish between these two protein isoforms. The monoclonal antibody Syn- I (Transduction Labs) has been frequently used for the specific detection of alpha-synuclein. In this report, the epitope for Syn-1 is localized within residues 91-99 of human alpha-synuclein. Sequence differences exist in this domain that account for the specificity of Syn-1 for alpha- versus beta-synuclein. However, Syn-1 also displays reactivity with additional species (similar to45 kDa) in brain homogenates from both wild-type and alpha-synuclein null mice, indicating a potential for cross-reactivity with a protein species that is unrelated to alpha-synuclein in brain tissue or extracts. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:133 / 135
页数:3
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