共 10 条
Epitope mapping and specificity of the anti-α-synuclein monoclonal antibody Syn-1 in mouse brain and cultured cell lines
被引:61
作者:

Perrin, RJ
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机构: Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA

Payton, JE
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h-index: 0
机构: Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA

Barnett, DH
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h-index: 0
机构: Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA

Wraight, CL
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h-index: 0
机构: Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA

Woods, WS
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h-index: 0
机构: Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA

Ye, L
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h-index: 0
机构: Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA

George, JM
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h-index: 0
机构: Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA
机构:
[1] Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA
[2] Univ Illinois, Med Scholars Program, Urbana, IL 61801 USA
[3] Univ Illinois, Dept Cell & Struct Biol, Urbana, IL 61801 USA
关键词:
synuclein;
immunoreactivity;
Parkinson's disease;
dopaminergic;
breast cancer;
knockout;
D O I:
10.1016/S0304-3940(03)00781-X
中图分类号:
Q189 [神经科学];
学科分类号:
071006 ;
摘要:
While alpha- and beta-synuclein largely overlap in their expression in the vertebrate brain, only alpha-synuclein accumulates in the fibrillar aggregates typical of Parkinson's disease. It is thus critical to have immunological reagents that distinguish between these two protein isoforms. The monoclonal antibody Syn- I (Transduction Labs) has been frequently used for the specific detection of alpha-synuclein. In this report, the epitope for Syn-1 is localized within residues 91-99 of human alpha-synuclein. Sequence differences exist in this domain that account for the specificity of Syn-1 for alpha- versus beta-synuclein. However, Syn-1 also displays reactivity with additional species (similar to45 kDa) in brain homogenates from both wild-type and alpha-synuclein null mice, indicating a potential for cross-reactivity with a protein species that is unrelated to alpha-synuclein in brain tissue or extracts. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
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页码:133 / 135
页数:3
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