Two-photon imaging of capillary blood flow in olfactory bulb glomeruli

被引：231

作者：

Chaigneau, E ^{[1
]}

Oheim, M ^{[1
]}

Audinat, E ^{[1
]}

Charpak, S ^{[1
]}

机构：

[1] Ecole Super Phys & Chim Ind Ville Paris, Neurophysiol Lab, INSERM, CNRS,FRE 2500,EPI 0002, F-75231 Paris, France

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2003年 / 100卷 / 22期

关键词：

D O I：

10.1073/pnas.2133652100

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Analysis of the spatiotemporal coupling between neuronal activity and cerebral blood flow requires the precise measurement of the dynamics of RBC flow in individual capillaries that irrigate activated neurons. Here, we use two-photon microscopy in vivo to image individual RBCs in glomerular capillaries in the rat dorsal olfactory bulb. We find that odor stimulation evokes capillary vascular responses that are odorant- and glomerulus-specific. These responses consist of increases as well as decreases in RBC flow, both resulting from independent changes in RBC velocity or linear density. Finally, measuring RBC flow with micrometer spatial resolution and millisecond temporal resolution, we demonstrate that, in olfactory bulb superficial layers, capillary vascular responses precisely outline regions of synaptic activation.

引用

页码：13081 / 13086

页数：6

共 52 条

[1] Functional recruitment of red blood cells to rat brain microcirculation accompanying increased neuronal activity in cerebellar cortex [J].