Diffusion imaging of whole, post-mortem human brains on a clinical MRI scanner

被引:208
作者
Miller, Karla L. [1 ]
Stagg, Charlotte J. [1 ]
Douaud, Gwenaelle [1 ]
Jbabdi, Saad [1 ]
Smith, Stephen M. [1 ]
Behrens, Timothy E. J. [1 ]
Jenkinson, Mark [1 ]
Chance, Steven A.
Esiri, Margaret M.
Voets, Natalie L. [1 ]
Jenkinson, Ned [2 ]
Aziz, Tipu Z. [2 ]
Turner, Martin R. [1 ]
Johansen-Berg, Heidi [1 ]
McNab, Jennifer A. [3 ]
机构
[1] John Radcliffe Hosp, FMRIB Ctr, Nuffield Dept Clin Neurosci, Oxford OX3 9DU, England
[2] John Radcliffe Hosp, Nuffield Dept Surg Sci, Oxford OX3 9DU, England
[3] Massachusetts Gen Hosp, AA Martinos Ctr, Boston, MA 02114 USA
基金
英国惠康基金; 英国工程与自然科学研究理事会;
关键词
Diffusion tensor imaging; Tractography; Post mortem; Human; Brain; ANISOTROPIC WATER DIFFUSION; SPINAL-CORD; VOXELWISE ANALYSIS; FORMALIN FIXATION; CEREBRAL-CORTEX; GRAY-MATTER; TENSOR MRI; WHITE; TRACTOGRAPHY; TISSUE;
D O I
10.1016/j.neuroimage.2011.03.070
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Diffusion imaging of post mortem brains has great potential both as a reference for brain specimens that undergo sectioning, and as a link between in vivo diffusion studies and "gold standard" histology/dissection. While there is a relatively mature literature on post mortem diffusion imaging of animals, human brains have proven more challenging due to their incompatibility with high-performance scanners. This study presents a method for post mortem diffusion imaging of whole, human brains using a clinical 3-Tesla scanner with a 3D segmented EPI spin-echo sequence. Results in eleven brains at 0.94 x 0.94 x 0.94 mm resolution are presented, and in a single brain at 0.73 x 0.73 x 0.73 mm resolution. Region-of-interest analysis of diffusion tensor parameters indicate that these properties are altered compared to in vivo (reduced diffusivity and anisotropy), with significant dependence on post mortem interval (time from death to fixation). Despite these alterations, diffusion tractography of several major tracts is successfully demonstrated at both resolutions. We also report novel findings of cortical anisotropy and partial volume effects. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:167 / 181
页数:15
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