Comparative chemical analysis of fiber material prepared by enzymatic and chemical methods from two mushrooms (Pleurotus sajor-caju and Pleurotus tuber-regium)

The fiber material of Pleurotus sajor-caju (oyster mushroom) and Pleurotus tuber-regium (tiger milk mushroom) was prepared by two protocols: the enzymatic approach based on the Association of Official Analytical Chemists (AOAC) total dietary fiber (TDF) method and the nonenzymatic method based on the cell wall material (CWM) isolation by the sequential use of chemical solvents. The gravimetric yield of the TDF of the two mushrooms obtained by the enzymatic method was higher than that of the CWM prepared according to the chemical method. The chemical method was more effective than the enzymatic one in removing the mushroom protein. However, some polymeric materials (including fiber material) were solubilized in the chemical solvents used to remove protein and starch from the mushroom. The nonstarch polysaccharide content and composition of the mushroom fiber obtained from the above varied depending on the morphology and types of cell wall polysaccharides present in the two Pleurotus species.