Caffeine releases a glucose-primed endoplasmic reticulum Ca2+ pool in the insulin secreting cell line INS-1

被引：24

作者：

Gamberucci, A ^{[1
]}

Fulceri, R ^{[1
]}

Pralong, W ^{[1
]}

Bánhegyi, G ^{[1
]}

Marcolongo, P ^{[1
]}

Watkins, SL ^{[1
]}

Benedetti, A ^{[1
]}

机构：

[1] Univ Siena, Ist Patol Gen, I-53100 Siena, Italy

来源：

FEBS LETTERS | 1999年 / 446卷 / 2-3期

关键词：

endoplasmic reticulum Ca2+ store; insulin-secreting cell; ryanodine receptor;

D O I：

10.1016/S0014-5793(99)00220-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Caffeine mobilized an intracellular Ca2+ pool in intact fura-2-loaded INS-1 cells in suspension exposed to high (16 mM) [glucose], while a minor effect was observed with low (2 mM) [glucose]. Cells were kept in a medium containing diaxozide or no Ca2+ to prevent the influx of extracellular Ca2+. The caffeine-sensitive intracellular Ca2+ pool was within the endoplasmic reticulum since it was depleted by the inhibitor of the reticular Ca2+ pumps thapsigargin and the InsP(3)-dependent agonist carbachol, No effect of caffeine was observed in the parent glucose-insensitive RINmF5 cells. In microsomes from INS-1 but not RINmF5 cells, the type 2 ryanodine receptor was present as revealed by Western blotting, It was concluded that the endoplasmic reticulum of INS-1 cells possesses caffeine-sensitive type 2 ryanodine receptors Ca2+ channels. (C) 1999 Federation of European Biochemical Societies.

引用

页码：309 / 312

页数：4

共 23 条

[1] ESTABLISHMENT OF 2-MERCAPTOETHANOL-DEPENDENT DIFFERENTIATED INSULIN-SECRETING CELL-LINES [J].