Hypoallergenic variants of the Parietaria judaica major allergen Par j 1:: A member of the non-specific lipid transfer protein plant family

被引:41
作者
Bonura, A
Amoroso, S
Locorotondo, G
Di Felice, G
Tinghino, R
Geraci, D
Colombo, P
机构
[1] CNR, Ist Biol Sviluppo, I-90146 Palermo, Italy
[2] Ist Super Sanita, I-00161 Rome, Italy
[3] Osped Civ, Unita Allergol, Palermo, Italy
关键词
Parietaria judaica; Par j 1 recombinant allergen; hypoallergenic recombinant Par j 1 mutants; skin prick test; immunoglobulin E; peripheral blood mononuclear cell proliferation;
D O I
10.1159/000049492
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background. Par j 1 represents a major allergenic component of Parietaria judaica (Pj) pollen, since it is able to induce an immunoglobulin E (IgE) response in 95% of Pj-allergic patients. It belongs to the non-specific lipid transfer protein family, sharing with them a common three-dimensional structure. Methods: Disulphide bond variants of the recombinant Par j 1 (rParj 1) allergen were generated by site-directed mutagenesis, and the immunological activity of rPar j 1 and its conformational mutants was compared with the use of the skin prick test (SPT). The ability to bind IgE antibodies was evaluated by Western blot, ELISA and ELISA inhibition. T cell reactivity was measured by peripheral blood mononuclear cell proliferation assay. Results: The disruption of Cys14-Cys29 and Cys30-Cys75 bridging (PjA mutant) caused the loss of the majority of specific IgE-binding activity. Additional disruption of the Cys4-Cys52 bridge (PjC mutant) and the latter Cys50-Cys91 bridge (PjD mutant) led to the abolition of IgE-binding activity. On the SPT, PjB (lacking the Cys4-Cys52 and Cys50-Cys91 bridges) was still capable of triggering a type 1 hypersensitive reaction in 9 out of 10 patients, and PjA in 3 out of 10 patients, while PjC and PjD did not show any SPT reactivity. All the mutants preserved their T cell reactivity. Conclusion: Recombinant hypoallergenic variants of the rPar j 1 allergen described herein may represent a useful tool for improved immunotherapy. Copyright (C) 2001 S, Karger AG, Basel.
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页码:32 / 40
页数:9
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