Genetic characterization of mutants resistant to the antiauxin p-chlorophenoxyisobutyric acid reveals that AAR3, a gene encoding a DCN1-like protein, regulates responses to the synthetic auxin 2,4-dichlorophenoxyacetic acid in Arabidopsis roots

被引:42
作者
Biswas, Kamal Kanti
Ooura, Chiharu
Higuchi, Kanako
Miyazaki, Yuji
Nguyen, Vinh Van
Rahman, Abidur
Uchimiya, Hirofumi
Kiyosue, Tomohiro
Koshiba, Tomokazu
Tanaka, Atsushi
Narumi, Issay
Oono, Yutaka [1 ]
机构
[1] Japan Atom Energy Agcy, Radiat Appl Biol Div, Takasaki, Gunma 3701292, Japan
[2] Japan Atom Energy Agcy, Adv Sci Res Ctr, Takasaki, Gunma 3701292, Japan
[3] Tokyo Metropolitan Univ, Grad Sch Sci, Dept Biol Sci, Tokyo 1920397, Japan
[4] Ehime Univ, United Grad Sch Agr Sci, Matsuyama, Ehime 7908566, Japan
[5] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA
[6] Univ Tokyo, Inst Mol & Cellular Biol, Tokyo 11130032, Japan
[7] Kagawa Univ, Inst Res Promot, Kagawa 7610795, Japan
关键词
D O I
10.1104/pp.107.104844
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
To isolate novel auxin-responsive mutants in Arabidopsis (Arabidopsis thaliana), we screened mutants for root growth resistance to a putative antiauxin, p-chlorophenoxyisobutyric acid (PCIB), which inhibits auxin action by interfering the upstream auxin-signaling events. Eleven PCIB-resistant mutants were obtained. Genetic mapping indicates that the mutations are located in at least five independent loci, including two known auxin-related loci, TRANSPORT INHIBITOR RESPONSE1 and Arabidopsis CULLIN1. antiauxin-resistant mutants (aars) aar3-1, aar4, and aar5 were also resistant to 2,4-dichlorophenoxyacetic acid as shown by a root growth assay. Positional cloning of aar3-1 revealed that the AAR3 gene encodes a protein with a domain of unknown function (DUF298), which has not previously been implicated in auxin signaling. The protein has a putative nuclear localization signal and shares homology with the DEFECTIVE IN CULLIN NEDDYLATION-1 protein through the DUF298 domain. The results also indicate that PCIB can facilitate the identification of factors involved in auxin or auxin-related signaling.
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收藏
页码:773 / 785
页数:13
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