Structural and mechanistic basis of immunity toward endonuclease colicins

被引:154
作者
Kleanthous, C [1 ]
Kühlmann, UC
Pommer, AJ
Ferguson, N
Radford, SE
Moore, GR
James, R
Hemmings, AM
机构
[1] Univ E Anglia, Sch Biol Sci, Norwich NR4 7TJ, Norfolk, England
[2] Univ Leeds, Sch Biochem & Mol Biol, Leeds LS2 9JT, W Yorkshire, England
[3] Univ E Anglia, Sch Chem Sci, Norwich NR4 7TJ, Norfolk, England
基金
英国惠康基金;
关键词
D O I
10.1038/6683
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The crystal structure of the cytotoxic endonuclease domain from the bacterial toxin colicin E9 in complex with its cognate immunity protein Im9 reveals that the inhibitor does not bind at the active site, the core of which comprises the HNH motif found in intron-encoded homing endonucleases, but rather at an adjacent position leaving the active site exposed yet unable to bind DNA because of steric and electrostatic clashes with incoming substrate. Although its mode of action is unorthodox, Im9 is a remarkably effective inhibitor since it folds within milliseconds and then associates with its target endonuclease at the rate of diffusion to form an inactive complex with sub-femtomolar binding affinity. This hyperefficient mechanism of inhibition could be well suited to other toxic enzyme systems, particularly where the substrate is a polymer extending beyond the boundaries of the active site.
引用
收藏
页码:243 / 252
页数:10
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