External quality assessment of molecular biology-based methods used in laboratories of clinical chemistry and human genetics

被引：11

作者：

Braun, A

Deufel, T

Geilenkeuser, WJ

Neumaier, M

Rohle, G

Roscher, A

Wagener, C

机构：

[1] Univ Krankenhaus Eppendorf, Klin Chem Abt, D-20251 Hamburg, Germany

[2] Univ Munich, Abt Klin Chem & Biochem, Dr Von Haunersches Kinderspital, Munchen, Germany

[3] Sequenom Instruments GmbH, Hamburg, Germany

[4] Univ Jena, Inst Klin Chem & Lab Diagnostik, D-6900 Jena, Germany

[5] Deutsch Gesell Klin Chem, Referenzinst Bionalytik, Bonn, Germany

来源：

CLINICAL CHEMISTRY AND LABORATORY MEDICINE | 1998年 / 36卷 / 04期

关键词：

external quality assessment; molecular biology; PCR; DNA preparation; DNA electrophoresis;

D O I：

10.1515/CCLM.1998.039

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The Reference Institute of Bioanalysis of the German Society of Clinical Chemistry has performed the first external assessment of molecular genetics methods used in medical diagnosis. The following procedures were tested: (I) DNA preparation from whole blood, (II) PCR amplification using "standard" primers, and (III) submarine agarose gel electrophoresis. Out of 50 participants, 45 returned samples for evaluation.

引用

页码：231 / 234

页数：4

共 9 条

[1] SEQUENCE AND ORGANIZATION OF THE HUMAN VITAMIN-D-BINDING PROTEIN GENE [J].