Isolation of fibronectin type III like peptide from human placental extract used as wound healer

被引：35

作者：

Chakraborty, PD ^{[1
]}

Bhattacharyya, D ^{[1
]}

机构：

[1] Indian Inst Chem Biol, Dept Drug Design, Kolkata 700032, W Bengal, India

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 818卷 / 01期

关键词：

human placental extract; fibronectin type III; HPLC; amino acid sequence; dot blot;

D O I：

10.1016/j.jchromb.2004.09.059

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A peptide of around 7.4 kDa has been purified from the aqueous extract of human placenta used as wound healer. Derived partial amino acid sequence from mass spectrometric analysis showed its homology with human fibronectin type III. Under nondenaturing condition, it formed aggregate, the elution pattern of which from reverse-phase HPLC was identical with that of fibronectin type III. Immuno-blot of the peptide with reference fibronectin type III-C showed strong cross reactivity. Since fibronectin type III plays important roles in wound healing, similar peptide in the extract is likely to take part in curing process. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：67 / 73

页数：7

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