Patients with ovarian carcinoma excrete different altered levels of urine CD59, kininogen-1 and fragments of inter-alpha-trypsin inhibitor heavy chain H4 and albumin

被引:50
作者
Abdullah-Soheimi, Siti S. [1 ]
Lim, Boon-Kiong [2 ]
Hashim, Onn H. [3 ,4 ]
Shuib, Adawiyah S. [1 ,4 ]
机构
[1] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur, Malaysia
[2] Univ Malaya, Fac Med, Dept Obstet & Gynaecol, Kuala Lumpur, Malaysia
[3] Univ Malaya, Fac Med, Dept Mol Med, Kuala Lumpur, Malaysia
[4] Univ Malaya, Ctr Prote Res, Kuala Lumpur, Malaysia
关键词
PHASE REACTANT PROTEINS; HUMAN SERUM; CANCER; EXPRESSION; COMPONENTS; DISCOVERY; PROTEOME;
D O I
10.1186/1477-5956-8-58
中图分类号
Q5 [生物化学];
学科分类号
070307 [化学生物学];
摘要
Background: Diagnosis of ovarian carcinoma is in urgent need for new complementary biomarkers for early stage detection. Proteins that are aberrantly excreted in the urine of cancer patients are excellent biomarker candidates for development of new noninvasive protocol for early diagnosis and screening purposes. In the present study, urine samples from patients with ovarian carcinoma were analysed by two-dimensional gel electrophoresis and the profiles generated were compared to those similarly obtained from age-matched cancer negative women. Results: Significant reduced levels of CD59, kininogen-1 and a 39 kDa fragment of inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), and enhanced excretion of a 19 kDa fragment of albumin, were detected in the urine of patients with ovarian carcinoma compared to the control subjects. The different altered levels of the proteins were confirmed by Western blotting using antisera and a lectin that bind to the respective proteins. Conclusion: CD59, kininogen-1 and fragments of ITIH4 and albumin may be used as complementary biomarkers in the development of new noninvasive protocols for diagnosis and screening of ovarian carcinoma.
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页数:7
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