Separation of proanthocyanidins by degree of polymerization by means of size-exclusion chromatography and related techniques

被引：62

作者：

Yanagida, A

Shoji, T

Shibusawa, Y

机构：

[1] Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Analyt Chem, Hachioji, Tokyo 1920392, Japan

[2] Asahi Brewery Co Ltd, Fundamental Res Labs, Ibaraki 3021016, Japan

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2003年 / 56卷 / 1-3期

关键词：

polymerized polyphenols; proanthocyanidins; size-exclusion chromatography; mass spectrometry; normal-phase chromatography; counter-current chromatography;

D O I：

10.1016/S0165-022X(03)00068-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular masses of polyphenols in plants and food vary greatly up to the order of 10 kDa. Polymerized polyphenols are not only natural antioxidants but also strong inhibitors of numerous physiological enzymatic activities. Several useful methods for the determination and separation of these high-molecular-mass polyphenols have recently been developed. In this review, details of the methods and applications of size-exclusion chromatographic separation of polymerized polyphenols, particularly those of proanthocyanidins, are described and compared with other related chromatographic or mass spectrometric analyses. (C) 2003 Elsevier Science B.V. All rights reserved.

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收藏

页码：311 / 322

页数：12

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