ZmMYB31 directly represses maize lignin genes and redirects the phenylpropanoid metabolic flux

被引:262
作者
Fornale, Silvia [1 ]
Shi, Xinhui [2 ]
Chai, Chenglin [3 ,4 ]
Encina, Antonio [5 ]
Irar, Sami [1 ]
Capellades, Montserrat [1 ]
Fuguet, Elisabet [6 ]
Torres, Josep-Lluis [6 ]
Rovira, Pere [7 ]
Puigdomenech, Pere [1 ]
Rigau, Joan [1 ]
Grotewold, Erich [3 ,4 ]
Gray, John [2 ]
Caparros-Ruiz, David [1 ]
机构
[1] Consortium CSIC IRTA UAB, CRAG, Barcelona 08034, Spain
[2] Univ Toledo, Dept Biol Sci, Toledo, OH 43606 USA
[3] Ohio State Univ, Dept Plant Mol & Cellular Biol, Columbus, OH 43210 USA
[4] Ohio State Univ, Ctr Plant Biotechnol, Columbus, OH 43210 USA
[5] Univ Leon, Dept Ingn & Ciencias Agr, E-24071 Leon, Spain
[6] IQAC CSIC, Inst Adv Chem Catalonia, Barcelona 08034, Spain
[7] Univ Barcelona, Fac Biol, Dept Biol Vegetal, E-08028 Barcelona, Spain
基金
美国国家科学基金会;
关键词
R2R3-MYB; lignin; cell wall; flavonoids; phenylpropanoids; maize; GLUTATHIONE-S-TRANSFERASE; R2R3-MYB TRANSCRIPTION FACTORS; CELL-WALL FORMATION; DNA-BINDING DOMAIN; ARABIDOPSIS-THALIANA; TRANSGENIC TOBACCO; DOWN-REGULATION; ZEA-MAYS; MOLECULAR CHARACTERIZATION; COMPREHENSIVE ANALYSIS;
D O I
10.1111/j.1365-313X.2010.04363.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
P>Few regulators of phenylpropanoids have been identified in monocots having potential as biofuel crops. Here we demonstrate the role of the maize (Zea mays) R2R3-MYB factor ZmMYB31 in the control of the phenylpropanoid pathway. We determined its in vitro consensus DNA-binding sequence as ACCT/(A)ACC, and chromatin immunoprecipitation (ChIP) established that it interacts with two lignin gene promoters in vivo. To explore the potential of ZmMYB31 as a regulator of phenylpropanoids in other plants, its role in the regulation of the phenylpropanoid pathway was further investigated in Arabidopsis thaliana. ZmMYB31 downregulates several genes involved in the synthesis of monolignols and transgenic plants are dwarf and show a significantly reduced lignin content with unaltered polymer composition. We demonstrate that these changes increase cell wall degradability of the transgenic plants. In addition, ZmMYB31 represses the synthesis of sinapoylmalate, resulting in plants that are more sensitive to UV irradiation, and induces several stress-related proteins. Our results suggest that, as an indirect effect of repression of lignin biosynthesis, transgenic plants redirect carbon flux towards the biosynthesis of anthocyanins. Thus, ZmMYB31 can be considered a good candidate for the manipulation of lignin biosynthesis in biotechnological applications.
引用
收藏
页码:633 / 644
页数:12
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