Fetal calf serum inhibits virus genome expression in Madin-Darby canine kidney cells persistently infected with influenza A virus

被引：10

作者：

Hossain, Md. Jaber

Mori, Isamu

Dong, Li

Liu, Beixing

Kimura, Yoshinobu

机构：

[1] Gifu Univ Med Sci, Dept Med Technol, Gifu 5013892, Japan

[2] Univ Fukui, Sch Med, Dept Microbiol, Fukui 9101193, Japan

[3] Aichi Med Univ, Sch Med, Dept Microbiol & Immunol, Aichi 4801196, Japan

来源：

MEDICAL MICROBIOLOGY AND IMMUNOLOGY | 2008年 / 197卷 / 01期

基金：

日本学术振兴会;

关键词：

influenza virus; gene expression; blood serum; virus carrier culture;

D O I：

10.1007/s00430-007-0054-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A cell line of Madin-Darby canine kidney (MDCK) cells persistently infected with human influenza A virus has been established and designated as MDCK-IVpi cells. Production of progeny virus in MDCK-IVpi cells was suppressed when the cells were incubated in the presence of 10% fetal calf serum (FCS). FCS impaired virus mRNA synthesis in MDCK-IVpi cells, which resulted in a scarcity of virus proteins for virion formation. However, MDCK-IVpi cells well supported the growth of superinfecting heterologous influenza viruses, even in the presence of FCS. A certain fetuin-like substance in FCS might be responsible for the observed inhibition of virus replication.

引用

页码：21 / 27

页数：7

共 39 条

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