Mutants of Pseudomonas fluorescens NCIMB 11671 defective in the catabolism of alpha-pinene

被引：18

作者：

Colocousi, A ^{[1
]}

Saqib, KM ^{[1
]}

Leak, DJ ^{[1
]}

机构：

[1] ROWETT RES INST, ABERDEEN AB2 9SB, SCOTLAND

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1996年 / 45卷 / 06期

关键词：

D O I：

10.1007/s002530050769

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pseudomonas fluorescens NCIMB 11671 metabolises alpha-pinene via alpha-pinene oxide and 2-methyl-5-isopropylhesa-2,5-dienal. Mutants unable to grow on alpha-pinene and/or alpha-pinene oxide have been isolated by N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis, including an unexpected phenotype able to grow on alpha-pinene but not on alpha-pinene oxide. The mutants have been classified on the basis of their alpha-pinene monoosygenase, alpha-pinene oxide lyase and aldehyde dehydrogenase activities. Biotransformation alpha-pinene by the wild-type and mutant strains has revealed evidence for alternative routes for pinene metabolism to that already proposed.

引用

页码：822 / 830

页数：9

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