The Coronavirus Nucleocapsid Protein Is Dynamically Associated with the Replication-Transcription Complexes

被引：80

作者：

Verheije, Monique H. ^{[1
]}

Hagemeijer, Marne C. ^{[1
]}

Ulasli, Mustafa ^{[2
,3
]}

Reggiori, Fulvio ^{[2
,3
]}

Rottier, Peter J. M. ^{[1
]}

Masters, Paul S. ^{[4
]}

de Haan, Cornelis A. M. ^{[1
]}

机构：

[1] Univ Utrecht, Dept Infect Dis & Immunol, Fac Vet Med, Div Virol, NL-3584 CL Utrecht, Netherlands

[2] Univ Med Ctr Utrecht, Dept Cell Biol, Utrecht, Netherlands

[3] Univ Med Ctr Utrecht, Inst Biomembranes, Utrecht, Netherlands

[4] New York State Dept Hlth, Wadsworth Ctr, David Axelrod Inst, Albany, NY USA

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 21期

关键词：

MOUSE HEPATITIS-VIRUS; RESPIRATORY SYNDROME CORONAVIRUS; N-TERMINAL DOMAIN; CRYSTAL-STRUCTURE; GENE POLYPROTEIN; VIRAL-RNA; INVOLVEMENT; INHIBIT; SITES;

D O I：

10.1128/JVI.00569-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The coronavirus nucleocapsid (N) protein is a virion structural protein. It also functions, however, in an unknown way in viral replication and localizes to the viral replication-transcription complexes (RTCs). Here we investigated, using recombinant murine coronaviruses expressing green fluorescent protein (GFP)-tagged versions of the N protein, the dynamics of its interactions with the RTCs and the domain(s) involved. Using fluorescent recovery after photobleaching, we showed that the N protein, unlike the nonstructural protein 2, is dynamically associated with the RTCs. Recruitment of the N protein to the RTCs requires the C-terminal N2b domain, which interacts with other N proteins in an RNA-independent manner.

引用

页码：11575 / 11579

页数：5

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