Genistein prevents the glucose autoxidation mediated atherogenic modification of low density lipoprotein

被引:45
作者
Exner, M
Hermann, M
Hofbauer, R
Kapiotis, S
Quehenberger, P
Speiser, W
Held, I
Gmeiner, BMK
机构
[1] Univ Vienna, Inst Med Chem, A-1090 Vienna, Austria
[2] Univ Vienna, Inst Mol Genet, A-1090 Vienna, Austria
[3] Univ Vienna, Dept Lab Med, A-1090 Vienna, Austria
关键词
glucose autoxidation; LDL oxidation; genistein; daidzein; soy phytoestrogen;
D O I
10.1080/10715760100300101
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hyperglycemia has been assumed to be responsible for oxidative stress in diabetes. In this respect, glucose autoxidation and advanced glycation end products (AGE) may play a causal role in the etiology of diabetic complications as e.g. atherosclerosis. There is now growing evidence that the oxidative modification of LDL plays a potential role in atherogenesis. Glucose derived oxidants have been shown to peroxidise LDL. In the present study, genistein, a compound derived from soy with a flavonoid chemical structure (4', 5, 7-trihydroxyisoflavone) has been evaluated for its ability to act as an antioxidant against the atherogenic modification of LDL by glucose autoxidation radical products. Daidzein, (4', 7-dihydroxyisoflavone) an other phytoestrogen of soy, was tested in parallel. Genistein - in contrast to daidzein - effectively prevented the glucose mediated LDL oxidation as measured by thiobarbituric acid-reactive substance formation (TBARS), alteration in electrophoretic mobility, lipid hydroperoxides and fluorescence quenching of tryptophan residues of the lipoprotein. In addition the potential of glucose-oxidized LDL to increase tissue factor (TF) synthesis in human endothelial cells (HUVEC) was completely inhibited when genistein was present during LDL oxidative modification by glucose. Both phytoestrogens did not influence the nonenzymatic protein glycation reaction as measured by the in vitro formation of glycated LDL. As the protective effect of genistein on LDL atherogenic modification was found at glucose/genistein molar ratios which may occur in vivo, our findings support the suggested beneficial action of a soy diet in preventing chronic vascular diseases and early atherogenic events.
引用
收藏
页码:101 / 112
页数:12
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