Effect of electric field induced transmembrane potential on spheroidal cells:: theory and experiment

被引:164
作者
Valic, B
Golzio, M
Pavlin, M
Schatz, A
Faurie, C
Gabriel, B
Teissié, J
Rols, MP
Miklavcic, D
机构
[1] CNRS, Inst Pharmacol & Biol Struct, UMR 5089, F-31077 Toulouse, France
[2] Univ Ljubljana, Fac Elect Engn, Ljubljana 1000, Slovenia
来源
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 2003年 / 32卷 / 06期
关键词
Chinese hamster ovary cells; electroporation; finite-element modelling; spheroidal cells; transmembrane potential;
D O I
10.1007/s00249-003-0296-9
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The transmembrane potential on a cell exposed to an electric field is a critical parameter for successful cell permeabilization. In this study, the effect of cell shape and orientation on the induced transmembrane potential was analyzed. The transmembrane potential was calculated on prolate and oblate spheroidal cells for various orientations with respect to the electric field direction, both numerically and analytically. Changing the orientation of the cells decreases the induced transmembrane potential from its maximum value when the longest axis of the cell is parallel to the electric field, to its minimum value when the longest axis of the cell is perpendicular to the electric field. The dependency on orientation is more pronounced for elongated cells while it is negligible for spherical cells. The part of the cell membrane where a threshold transmembrane potential is exceeded represents the area of electropermeabilization, i.e. the membrane area through which the transport of molecules is established. Therefore the surface exposed to the transmembrane potential above the threshold value was calculated. The biological relevance of these theoretical results was confirmed with experimental results of the electropermeabilization of plated Chinese hamster ovary cells, which are elongated. Theoretical and experimental results show that permeabilization is not only a function of electric field intensity and cell size but also of cell shape and orientation.
引用
收藏
页码:519 / 528
页数:10
相关论文
共 39 条
[1]  
[Anonymous], 1973, MATH HDB SCI ENG
[2]   GENERATION OF POTENTIAL DIFFERENCES ACROSS MEMBRANES OF ELLIPSOIDAL CELLS IN AN ALTERNATING ELECTRICAL FIELD [J].
BERNHARDT, J ;
PAULY, H .
BIOPHYSIK, 1973, 10 (01) :89-98
[3]  
Bier M, 1999, BIOELECTROMAGNETICS, V20, P194, DOI 10.1002/(SICI)1521-186X(1999)20:3<194::AID-BEM6>3.0.CO
[4]  
2-0
[5]  
Brock T.D., 1985, Biology of Microorganisms, V4
[6]   Electrooptics studies of Escherichia coli electropulsation:: Orientation, permeabilization, and gene transfer [J].
Eynard, N ;
Rodriguez, F ;
Trotard, J ;
Teissié, J .
BIOPHYSICAL JOURNAL, 1998, 75 (05) :2587-2596
[7]   Modeling assemblies of biological cells exposed to electric fields [J].
Fear, EC ;
Stuchly, MA .
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 1998, 45 (10) :1259-1271
[8]   A mathematical treatment of the electric conductivity and capacity of disperse systems II. The capacity of a suspension of conducting spheroids surrounded by a non-conducting membrane for a current of low frequency [J].
Fricke, H .
PHYSICAL REVIEW, 1925, 26 (05) :0678-0681
[9]   Fluorescence imaging in the millisecond time range of membrane electropermeabilisation of single cells using a rapid ultra-low-light intensifying detection system [J].
Gabriel, B ;
Teissié, J .
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1998, 27 (03) :291-298
[10]   Analytical description of the transmembrane voltage induced on arbitrarily oriented ellipsoidal and cylindrical cells [J].
Gimsa, J ;
Wachner, D .
BIOPHYSICAL JOURNAL, 2001, 81 (04) :1888-1896